XB-CLONE-3555558
Clone Name: IMAGE:7713379
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Gene:
abce1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971718 [+]
ctttatctccctccttccccccagtgtttaacatttccgccgtcacccatagctgaatatcttttcctgcggtgttaaagttggtgtccattgaaaaaca
aagctacagttataccagacaatatggcagacaaactaacgagaatagctattgtcaatcatgacaaatgcaagccaaagaaatgtcgtcaagaatgcaa
aaagagttgccctgttgtaaggatgggaaaattgtgcattgaagtgactccacaaagtaaaattgtatggatttccgaaaccttgtgcattggatgtggt
atctgtatcaagaaatgtccctttgtagcattgtcaattgtcaacttgccgagcaatctggagaaggaaacaacccacagatattgtgccaatgccttta
aacttcacaggttgcctattccccgacctggagaagtacttgggttggttggtacaaatggtatcggaaaatctacagcattgaaaattttggctggaaa
gcagaagccaaaccttggaaagcatgatgatcctccagactggcaggagatcttgacctatttcagggggtcagagttgcagaattacttcaccaaaatt
ttggaggatgacctgaaggccatcattaaacctcagtatgtggaccaaattccaaaagctgcaaagggacctgtgggtaccatattggatagaaaagatg
agacaaagacacagaaatctgtatgtgatcaactagaccttactcatctgagagacagaaacgtggaggatctttctgggggagagctacagaggtttgc
ttgtgcggttgtctgtatccagagagc
3' EST: CX971717 [+]tcaaaccttgaagtttaggctctctttaaaataagatcatgttctttatggcagcattgtatgtaaaccatcaaagaaatccagtttacaaaggtctaaa
attggggacaattaatctgaatattgaaaagtgaataaattagattgccaggttctcaatccatacaataaattgtactttgcactttttatgaactttt
cttatgtggccaatcacaacagttcttggtcagttttactttatcctgcaacataatgcagaaggttgtatgaaacaatgtttcatacatcagagaggct
tgatttcttcggttgtagaaaggtgtgggttttagcttagtcatctaagaaaaagtagttgccactcttcttctgatccacatccttaatagaattcatt
ttgtttattcttggtctgtagttgttaggatctctcctaaacgtgatttccagctgtgacaagaacttgttcatgccagccaatagtgtttgagggctgt
tagcaacagtatctttggaaggtataccatcaaacacaatgactctgtcagcaagataagtcgccattataaaatcgtgctcaacaataaaagccgtcct
cttggcgtggagaatgaatcttttgattactctggcggctgtgagacgctgttcagaatccagatatgctgatggctcgtcaattagatacacatccgca
gctttacctaaacacagagttaaggccaccctctgtaactc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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