XB-CLONE-3555508
Clone Name: IMAGE:7713527
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Gene:
phrf1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971751 [+]
agaaagaaaagaaaaaggagaaaaagaaaaaaagcacagattccaaggaaagaaagaggtcaaggtccagtagtcggagcagctcttcccacagaatgta
caacagcaaaaagaagaaaaaaaagaagcgtggatccaggtgtggttccaggggccgttcttttagccctgaaaaggacaagaagcgaaaacacaagtct
gagaaaagttgttccactaaagatggggactctagagtaaaagagaaaaagaaatctaaggaggacagggggaaacacagatccagttcgccacctacaa
tcaaggagcaaaagagtcatcgaactaaagataaaaatttgcgtcatagagatcaggaactatccaaagattctttgcattcaccagaaagagaggaaaa
gacactaacgtgtaccgtcagtgttagtaaagactttcaccctgtcaagcatgaggcaaggaaatacacattaattgtacccaaagaggaggttgaaata
gaatccacttctgctttggagagtgacatttcaacaccagttaaggaggaaagagatattgacttatcaaacaatgatgttgaagacgattatgaaagcc
aagtagaaaacaatagcagcccaccatggtctccatcactcttagattccttacttcctgaggacaaacatgatgatgttgaagaagtgcaatctctgga
ggatattgattcatcaaatgacttgcttcctaatgcagaggtagctagtcctcaagcttctccattgactacagatactgaacctcccagtccacaacct
accagtccatactcgagtccttttccaaaaaaagatggactcaaaactccctcagtggtgaaaaca
3' EST: CX971750 [+]aaaaattctggcaattgttgtcctttatacatattttagggtcaaaggacaaaatctttatttcagaatgtattctttcatcgccctgcatttgttgcgc
catgtccccctcttacttcctttgcttgccatgcgaaaaaaaaaaaatctgatgcacggcttaacaaaaattcctttctcttacaaatgagtttacttgg
tcctccagggagacgttctcaacacagggaccgccaagatggcagcagggtttccgaacaagttgttgattgatgtaaaaaatagggggcaatcttgatt
tcttctttttccccctctatgtttcacaccttgaaacttctgcataggcaggaagataacctctttttgctggcagagggccctgccatctgtacactga
agtccaaagaaaccaaactgggactggtgaaaacaaaggattgggtgtcttgaaatgagcagaacatccccttcatgtacgaaactaatgatcaacttca
actgcacaggcggagccccatgcagcccaactctgctgcaccgtcgctgaggctgaacacttttacatctgttttcatacgacaaagacttttcttctca
tagtggagaatcacgcatagtcccatggtcctcaaacgactccgacttcttgtgttttcgtgcgtgtttgtacttatccacgtaggcttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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