XB-CLONE-3555491
Clone Name: IMAGE:7713433
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Gene:
arrdc4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971762 [+]
tcatgcctttaaaagggaacacggaattttaagggaattctacaaactatttgatgtggcgcattgattggtgctcgctaccatttcctacagtatttgt
aaatataaaagaaaatgtgagctttatgcaagttggtagtgatacaactacataatactgccaaaggtcctgttgcacatgatcagtctttgtcttgctt
tgttaaatttgtcttactcctaataagaaagaatagagaaatctagaaggacagtttgagagaagcagagggacaaacaggagagaaagagaaggaagcc
tggactagagggagaggtagaggaacattagatagagcagcagaaggactggtaagagagagagagagaggtggattaataagccagagctcatgagaga
agaaaagggactggctagacagaaaggatgcctcacagagagattatagaaagaggtggaaaggcaaaataaagaacagtggggagtatgtaaagaacgg
ttaccctaacggctcttcaggttgagacacagacaaaaagtgagagagtatactaattataactaatattttctttgtggttttctttcctctataactc
acactatgggaacttcaatttaactcttaatgcagacgttttgattttgaagagatactctagtttttgctgcctgtattatgtggcatttattgtttgt
ggcagttcaggagtaaaacatgtgctgttccttttttttatggtaccttgtgtacatacatatagtgccatgataaaacaaatggtatatacccattaaa
ctaaatactaaatccactaatgcagtataataaatacctaatg
3' EST: CX971761 [+]tacaaacaccatcttgtttttttgttatcaaaacagaggcttgtaagagaaatgtgcaaatagaaatgcatagggtttaacaacaagatatcaaagaggt
acatttcgtacatttataaaacaaaacatcccatttaatggggaaatgtaaggccacgatttgatagagattacagcaggcatatgtatgtagatatgta
aaaataataacatggttactatccaacaggtctctttaaagagaatgcttgctattatataaaagccgatacagtgcatgtttaatattattttacagta
gtctaataatgcgtaaattcctcagcctaacttattttggactacacgtatgataatcccagcataagtattgcagctactgatgtactggctgtactgt
ctgcactaggacacaatgttaggctgaagaatgctaattaatgtttgtatctcacaaacgatatggaaacaaatacttgtgaaatatatatccccccagt
ttagatgcatgcttatattaatgacagctctcctttaatccctgcaggaccacacaatgtatatattagtggaatacatgtctgtatgaaaggcacccta
cattttgctttgtatcccaaataaatcagtttcataagatgcactgatatcaaacgcaagctagctatttcacttttaaccagtttggatttggaataaa
tattaataaagactcaaagaaggacggaatcgttacagcaccttaaactatttaggcacaaagggccacattcagttta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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