XB-CLONE-3555367
Clone Name: IMAGE:7713636
|
Gene:
cel.2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX971847 [+]
caaatttataaacgtttgtgcccagaatctgacgtagggagaacgcaagctctgcttcatggactggtagtttatcttgtggttgatttccaggtattgg
ccattctcagttgagtaggctagccagggggtaggaatgctggattcgcctttactggggtctccagtcgccgcaaagttggtccaatatgcaatcatgt
actgggccacatctctgtctctgggtctgtaagccagcggggtggagaacggtttgccaaacataaactgcagatcttcggcgtggtcagcccccatcca
gctggggtaaacgggcatacggcttgggtggctgaacatatagctgtatgtcttggcgcccttggcattctggtagtgtagcgccagagcctcctgagta
gccaccaggaagatatagtctgtttccacttccacgaccgtcctcttcatgtcttcttgactgggattggtcccccagccggctgtgtaaagatcgtacg
caatatcgagtgccggcacccctttggctagggttaagccccgcaccactttctggacctgttcggtgctgattttgtggagaggctggtttatagcagg
caaatcgacgccggcaaaaagatgtccatccatgttgtttacccccgctatgtagtcgacccccgctgcgttggagaataggttcctgggttcatcaggg
atgaagtctccatctatgaccggngagtaggccaagtaatggactanaggatattcaaggttaaacagttgcagctgataggctatagtgagggcgcctg
ggtctgtgtttcgcaagcaatctgccagctcttt
3' EST: CX971846 [+]ggtggcccatgccaaacgctcggcgtagttcacaccgaagggggatttgttgaaggaaccaataagaagattggggttgtcttccccgattacattgata
ttttcaaagggatccctttcgctgctcctcccaagcccttcgaaaaagctgagaagcacccgggatggtcaggtaccctacaggctaaagactacaagcc
gcgctgtttgcaagccaccatcacccaagacaatgtcttcggaagcctagattgtctctatctgaacgtctgggtgcctcagacccgctctcaagtgtcc
acaaatctccccgttatggtatggatcttcggaggggggtttttacttggcagcggtcaaggagccaacttcctgagcaactacctgtacgacggggaag
aagttgccatgcgcggcaaggtcattgtagtgaccttcaactaccgcgtgggacccttgggatttctcagcactggcgactctaatgctcctggtaacta
tgggctgtgggatcagcatatggccatcgcctgggtgaagaggaacattgcagcatttggcggggacccgaacaacatcaccctgtttggagagtcagct
ggaggtgctagcgtttcactccagacactgaccccgtacaacgtgggactgattaagcgggccatcagtcagagtggcgtgggcctgtgcccctgggcca
ttcagcgggaccctctcacttgggccaaatcgcttgcgtcaaagctcgggtgccctgtgaataataccaaagagctggcagattgcttgcgaaacacaga
cccaggcgccctcactatagcctatcagctgcaactgtttaacctttgatatcctttagtccattact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: