XB-CLONE-3555306
Clone Name: IMAGE:7713546
|
Gene:
hla-dra
,
mhc2-daa
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX971887 [+]
cagaaacgaggggcctggcagaccgatatctggcctaaaattggccggatatcgatcggccaggttagaaaatccagtcggatcggggaccgcatcgact
cgttgatgcggtccccgagccgactgccccattgcccgtaggtgcggatccggtggatctgcccgtgtatggccagctttagtctgtaatgcaggcaatg
taacttgaggctcaaagcagagaatgttgtaatgcttatagctctgatatttttatgaatcatacttcactgaaataatattgtaaatttcccctcccaa
tgcaatatcattttcttcaaattggaaatccaatgtatttacctgttcatttaaccttaattatcccatcccaaaccattcttgtacttaattctgttta
gagaagggcatactgatagccagcaaagatcatcagcactgggttatctgaaaggcactcacataatgccagaagctgcagcattacccagacatttttc
cactgacactcagcaatttgactgcagtaggaagtttctggagacttgaattaaactcaaagaaaagtctctggtctcattctgaaataactgtatctat
attctgcatggctggaggcatttttttcttttatttcttaaagggataagtttaacaaatgcttattttcagtacaactgtacaataacaatgactattt
cagttccttcctactagatcctattaaatcccatagatttaagttaaacttcctccctctggtgtttgtccactacactataaacagacagtctgaactg
ttacaatttcctacatttgtgatacatctttctgaagc
3' EST: CX971886 [+]taatgcataaggcagaaggcacaaaactaaagaaaatgtcctacgtgagatttaaacagcactctcttataagatataaatctcttaagatatagttctg
tcttcaatcatgtgttgcattccagctaccgcagggttacaagcattattactatactgatcatctctacagaaagacaaaagttaaatgaattataggg
ttggggttacactttttaatagctttatttttgtttaggagatcaagaaaaacagcagggcatccatttagtttgtacagaaagtagcaatcaagatcag
ggctatataagaaaatacaagaaagtacatgactggatagaatactttgcagttctggagctaccagaccatagagcaagccaattaacagaggatgtta
caacttcaacattcaataacgatcctccttgtagctgggggtaatttgatattcattcaaagccaacacatacattgtattttaaatcatcatcaaataa
aattgtgggttagaatgatatacatcacacagaaaccattgcaaatattgaggctgcaattaaatgtaattttcaatttcagagatgcatctaaaagcta
agctaatttaaaaacaaaaaaatcaaggaatcaatctttcatacactgtagcagagattatagtatgtgtgatgcccaatgatatatgagtgctatttca
tggtgaatatgaacagtttctattctgtttgtgtaagcaaggtatattcagcaaaacaaaggagaacagatacaccacattaaaagaatacaaaattgtc
ccactgatttgcataccaccctcaagcactagtataagcacttgtcaggtgacagctggaaacatgaactgccatgattttgtgttacttaacacctta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: