XB-CLONE-3555268
Clone Name: IMAGE:7713406
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Gene:
lct.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971912 [+]
caacgatccaagggatgtggaagccgctgaccgatacctacagttcacactagggtggtttgcccaccccatcttcaaaacgggggactacccagatgcc
atgaaatggcaagtggcgaataaaagtgaattacagaaactgaaatcctccaggctgccaagcttcaccgataatgaaaaagcctacatccaaggcactg
ctgatgtcttctgcatcaacatctacacctctaaaatagtccagcacaaaacaatcaagctgaaccctccatcctttgaaggagacagagatacacagga
atcatttaactcaaactggccaacaactggggtttcagagcacagagaggtgccctgggggcttaggaggcttcttaactgggtcaaggaggaatatgag
aatgtgcccatttatattactgagaatggcgttaccataagcactgccactgagttcgatgacaacaaccgcatcttctactacaaaacctacattgatg
aagcactgaaagcaatcaaacttgatggggtgaatttgaaaggttacacggcctggtccctcatggacacctttgaatggaatttcggctatagcccgcg
gttcggcctgcaccacgtggacttcacaaatcccagccgcccaaggacccccaaacattccgccatctactatgcagaaatcatacggaacaagggcatg
ccccttagcaaggaggacgagttcctttatggggagttcccaaaagattttgcatggagtgtggcatctgcttcctaccagattgaaggtgcctggagag
ccgatggcaaaggcctgagtatct
3' EST: CX971911 [+]tagggcgagaggccaaaaacccacatttcaagctgaaaggcttagatactggagatatttacagcattgccgtacagtgatttctgctcagaatggaaaa
tatggcacttaattagatgaccagggctaagtggctgatcacttggatcagtacagtaattacccaatgggcctgcaaagcctagtctgtatactggggt
tctgtctacttacacattgttaccaggtacgtggcaccactgagccctggggggttggcatgggaagtggcgctcactgcagggagtcggaatggtctct
gcccaataaaactttattctggtttctaaagcaaaacagcacatttccttggtattatgcacccgggggcaaccctggaatcatggcgtccatacacttt
ggcatcatacgatcacttaaaagctgctcactttcattgctttctttgctaatttgctgtagaaaatggaaaacaagatgattcctaaaaccgctgcaat
cagaatgccaaattccacgtagagggcagtctgggcatctgagggtgaaatctccagcccaaggaaggaaacggccgtatcttgaatttcaccggaaggt
ggtttggtgagggtgggcgtgctgtgggtgagggtgggcgtgctgtgggtgagggtgggcgtgctgtgggtgagggtgggtgtaggagcggttgtgcctt
ctgcctcgggctccagacaaggaggggtgccattacttgngtcagggaagccattgcaccgaatgatagatctgtagtactttgccgactctttagggat
gcgtgccaaattcagatccgagtagttggtgtaatgcaagccaaagcgttcggcaaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: