XB-CLONE-3555238
Clone Name: IMAGE:7713504
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Gene:
cd74
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX971932 [+]
cagaacttggtgccggagcatgttcccgaagaaagtgtggtcgatgtcggaaacagacagccacggatgtcctgtaacaaagggagcctggtgacagctc
taacggtgcttgtagccgtgcttgttgccggacaagccgttatggcctatttcatcacccagcagaattccaagattcagaaacttgaccaaaccaccaa
acacttgcagctgaaagatatgatgaagaatcttccaggatcgcctcctgcccaaattaaatcaaagatgagaaccttcaatatccccatggcgttaaaa
ctatatgatggctcagaaacaaatatgaatgaactggagcatctggctcagatcgataacaaagtggaagatgctgcaaaatacatgttgctgagaggaa
accctctaaggaagtacactttaccaaatggcactatcctggaaaacctgagagagctgaagaagtccttgactgaccaggaatggatgaactttgatgc
atggatgcatcagtggtatctcttcttcttggtgcaaaacactggaaaggcagctgaacccctcccaccgcaaaagaatattgcagtcacaggtgccccc
ctgatgactgagtgtcagacattatcaaggatccccaccctgactggcgcttataagcctcagtgtgagcaaaatggtgactttaagccgaggcaatgct
ggccaagcactggctactgctggtgtgtttaccgtaatggaactgaggtccctgactccagaacccggtggtcaagacctgcctgcagtgatgtcatgga
gccagaagaaccatttttctgggatcaacacccagcgatgactttaatcccattgata
3' EST: CX971931 [+]tttttttaagatgtaactgctcattttattaggcatggaataaggtatgtgcacgttatacagaacctgtaagtctgacagaagccgcaattaacagcag
agaaaaagttaaaaaaaaaataaaaatggcagccatatttctatcaaaacacaattagcgataaatgaatgaacaacgattttatcagtgcccatttgaa
ttgtcaaatcatgttaatgttatcacattccccagaccgtaccttactgagacctccttaaccatacagaaactactagatttgttagtagtggcaagac
gtggaggatttgtccatggtatagagacatagacaagccacttttcagaagtactagttagtcagcctcctggtagatacttcctttcaatcatgtgcag
ctacaatcacagtgggactgatctgtgtaaccataccacttattattcctttaacatgctgctgtaacacacactgtaattcataaaaacaaacaaagta
ctaaacactaccttaagcctattgagaaacctgaggccagaatgccatagcaattagtaacaacataaagtcagattaataggaatcctcttgtgctgct
tgtatgaatcagggtagatactgcagaggaggttacgtgctgatccgatgctttaatcaatgggattaaagtcatcgctgggtgttgatcccagaaaaat
tggttcttctggctccatgacatcactgcaggcaggtcttgaccaccgggttctggagtcagggacctcagttccattacggtaaacacaccagcagtag
ccagtgcttggccagcattgcctcggcttaaagtcaccattttgctcacac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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