XB-CLONE-3555215
Clone Name: IMAGE:7713554
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Gene:
afg3l2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125787 [+]
GCACTTTTAGGCAGACGCAAAATCTATTGGTGTTGTGCTGCGGGTACTGAATGGCGCATCGCTGTCTGCTGCTCCCCTGCGCTGGGCGGGCACTGACGGG
GACCCTTGTACAACTTCTACCCCGACCTGCTACTGGCAGAGCAGGGGCCTGCTGTTTGGGCAGAATTCACACCCAAGTAAATTCATTTGGGAAAGAAAAG
ACATGGGCCAATATTCTAGCTGCTTACAGAAGACTCACTTCTCAACCCCCAAAAGGTTTTGAGAAATATTTCCCTAATGGCAAAAATGGCAAGAAAGCCA
ATGATAGCAAGGGGACATCAAGTGGTTTAAAAGATGCCAAACGAAACGATAGCCAACAGTCCACAGGAGGGAACAGTAATTCTGGTGGAGGGGGTAAGCG
AGGTGGAAAGGGAAGTGGAAAGAAAGAAGACTCAACATGGTGGTCAAGATTACAGAAGGGAGAAATTCCATGGGATGATAAAGAATTCCGGTTCTATTTT
ATGGCAAGTGCTGGTTTTTGGGCCAGTGTTACCTATTATTTCTTCTTCAGAAACTCTGGCAGAGAGATAACTTGGAAGGACTTTTTCAATAATTACTTAT
CTAAAGATATGGTGGACCGCCTTGAGGTTGTTAATAAACGCTTTGTTAGAGTCATATTTACGCCAGGAAAGTCACCTGTTGATGGGCGTTATGTCTGGTT
TAATATTGGCAGTGTGGACACTTTTGAGAGGAATTTGGAAACTGTGCAGCAAGAGCTTGGTATAGAAGGAGAAAACAGGATACCAGTTGTGTATTCTTCT
GAAAGTGATGGCTCCTTCCTGCTGAGTATGCTTCCTACAGTACTGATAATTGGCTTTCTCCTGTACACACTGAGAAGGGGGCCAGCTGGAGCAAGGCAAG
GCCGTGGTATGGGTGGCTTGTTCAGTGTTGGAGAGACAACTGCAAAGGTGCTCAAGGATGAAATTGATGTAAAGTTCAAGGATGTAGCTGGATGTGAAGA
AGCAAAACTCGAGATAATGGAATTTGTTAATTTCCTGAAAAATCCAAAGCAATACCAAGATTTAGGTGCAAAAATACCAAAGGGTGCTGTTCTGACTGGT
CCTCCTGGCACAGGGAAGACTTTGCTGGCAAAAGCTACAGCAGGAGAAGCCAATGTCCCTTTCATCACAGTCAATGGCTCAGAGTTCCTAGAGATGTTTG
TTGGTGTTGGGCCTGCCAGAGTGCGTGATTTATTTGTACTGGCAAGAAAAAATGCCCCTTGTATCCTATTCATTGATGAGATCGATGCAGTTGGTAGAAA
AAGAGGACGTGGAAACTTTGGTGGTCAAAGCGAGCAGGAGAACACACTCAACCAGCTCCTGGTAGAAATGGATGGTTTTAACACAACAACAAATGTAGTT
GTTTTAGCTGGCACAAATAGACCAGATATTTTAGATCCCGCACTCTTGCGGCCTGGGCGGTTTGACAGACAGATCTATATAGGTCCTCCAGATATTAAAG
GCAGAGCATCTATATTTAAAGTGCACCTCAGGCCGCTAAAGTTAGATGGAGCTATTGAAAAAGAAAGTTTGGCAAGGAAGCTTGCTGCATTAACACCTGG
TTTCTCAGGAGCTGACATTGCGAATGTGTGTAATGAAGCTGCTCTCATCGCGGCAAGACATCTTTCTGATGCCATAAACCAAAAGCATTTTGAGCATGCC
ATTGAGCGGGTAATAGGAGGTTTGGAAAAGAAGACCCAAGTTCTTCAGCCAGAGGAAAAGAAAACCGTTGCGTATCATGAGGCTGGCCATGCAGTGGCAG
GGTGGTTTCTGGAGCATGCAGATCCTCTTTTAAAAGTTTCTATCATTCCAAGAGGCAAAGGATTAGGTTATGCACAGTATTTGCCTAAAGAACAGTATTT
ATATACAAAGGAGCAGCTGCTGGATAGGATGTGCATGACACTGGGAGGTCGTGTATCAGAGCAAATATTTTTTGGAAGAATTACAACTGGTGCACAAGAT
GATCTGAAGAAAGTTACTCAAAGTGCCTATGCCCAGATTGTGCAGTTTGGAATGAATGAAAAGGTTGGCCAAGTGTCTTTTGACCTGCCACGTCAAGGAG
ATATGGTGTTGGAGAAGCCTTACAGTGAAGCAACTGCCAGACTGATTGATGATGAAGTTCGAGTTCTTATTAACACCGCTTATGAGAGAACATTAACGCT
TCTAACAGAGAAGAAAGCAGACATTGAAAAGATTGCCCTTCGCTTGCTGGAAAAAGAGGTGCTTGATAAAAGTGACATGGTTGAATTACTGGGCAAAAGA
CCTTTTGCAGAGAAGTCAACTTACGAAGAGTTTGTTGAAGGCACTGGCAGTTTGGATGAGGATACTTCCCTCCCTGAGGGTCTAAAGGACTGGAACAAAG
ACAGAGAAAAAGAAAGGGATGATTCTTCTGACGAGCAGATTACCCGACAGATTGGTGGGCCGATGCCCTTTTGATCAGTTATACAATTTGACTTGTAGCA
CTTTTTTTCATTATGCTGGAACTCTGGTCAACACAGATGAGAACAGGTGGTTTCTGATAGAGGGAAAACTTCTAAGAATAAGGGATGTTTTAAAATGTTT
ATTCAAGCAATTCTTATGTTCTTTAAATCTTTTCTTTCAGTATGAATTGATGAATGCAAGTATTGCACCCCTCCCTGCATATGTGACATTTATGCACAAG
ACTTAAGTTATTATGCATTTTTTACATTAAATGCTGCTGTGTCATAATGTCACTGCAAAAAGACCCTCTAATATTGATGTTCATTTTATATGGGGAATTG
TGTTATTGGTACACATTAATTTATGATAAGATGGTAAGGAGGTTGCAAAATGACATAGTAGCCTGGGCTACAAAATGACTTTCTTGTTGTGGCAGATATC
TGTACACTCTTGTGCAATCTGTAAAGAATTTGTCTTGTTTGAAATTAATTGCCTCTGTGGAATCCAAGAGATCAGAAAATGACCCAAAAATGATGCAAGT
TCTGCTATCGTAAGGCAGAAGCATCAGCGAGAGGCATGCCATTAACCCAACACAGAGACCTTCACATTTTCAGCTCTGGGGATCATTTCCTGACAGGCAC
CAGGGATAGTTCTGCACATTGTGTTAACTGGTAGTTCACAGCAGCTGCTTCCAGTATTACAAACTAAATAATATATTTGTTGATGTGGAAAAAAAATGTT
GTTTTAAAACAACTTAATGACATTTGGGACTGCATTTCTAAGATGAATTGCAGTTTACAAATATTATATTGATGATGACTAATGTGCAAGAAAATTATGA
TTTATGTAAGTATTTATGCCTACTATCCAAAATCAAACAGAATGCACACAATACAATCGTCTGCTGTTTCCTTTTATTCATGGGTTGCCAGTGAGCTTGG
CAAGAAAACTGCATGCTCTCTCTGTAACATGTTCCAATGTAGCCTATGAAATGTCACCCTACGCCAGTCAGTGTTTTTAAACAGATATCTACAATTTTTT
TGAGATTTTGTGATGTGATATGTATTCTGCCTTCCACAACCAGGGCACATACTAGAATACTATGTCTTCCCATCCCTGCAAAGCCTAAACACCATACAGC
ACAGTAAAATAATTTAAAATAAGTCTAAAAGGAATAAGGAAGGATTAAAGGTATCTATGGGGCAGTTTATTTTGCAATATTGTCGATTTTATCCAAAGGT
TAGATTTAAGTTCGTTTTATCCAACCCTATGCATGTTTATGTACTATAACTCCTTAACATTGTGGGTACTGGGAACCTTTTGATACGGAGTTCAAAAACA
TTGCATCTTAACTTACCAGGCACAGTTCGGTCAGTAAGCCTTTGTTTTCACTTGTTATATGGAGCTTGTAAACCTGTGACTGAGACTGAGCATCTTGGAC
TCAAATTATAGGTCCCACAGAAATGGCCTTTTATTCATATTTTTAAAGGACCTATAATGAATCATCATTTTTTTATTATATAAATATTACAGCAGCACTG
AATGTACATGTAGATGTACTGTAGCACTTACCTGGGTCTTTTCCTGTAAGAACTTAATTGTGGCATTACTTGTGGTTGTACTTTTAACACTTTCACTGTA
CGATGCTTGCCAAGATGTACCGCAAATCCAGTTATGGCTGAAAGTTTGAGTTTTACCAAATTGCACGGAATATATTATAGTGAAATTGGTAAATGGAAGC
AGGTCATATATAGATAAGCACTTAATTTTGATGAAATACAAACCCTAAAATTT
5' EST: CX971947 [+]cacttttaggcagacgcaaaatctattggtgttgtgctgcgggtactgaatggcgcatcgctgtctgctgctcccctgcgctgggcgggcactgacgggg
acccttgtacaacttctaccccgacctgctactggcagagcaggggcctgctgtttgggcagaattcacacccaagtaaattcatttgggaaagaaaaga
catgggccaatattctagctgcttacagaagactcacttctcaacccccaaaaggttttgagaaatatttccctaatggcaaaaatggcaagaaagccaa
tgatagcaaggggacatcaagtggtttaaaagatgccaaacgaaacgatagccaacagtccacaggagggaacagtaattctggtggagggggtaagcga
ggtggaaagggaagtggaaagaaagaagactcaacatggtggtcaagattacagaagggagaaattccatgggatgataaagaattccggttctatttta
tggcaagtgctggtttttgggccagtgttacctattatttcttcttcagaaactctggcagagagataacttggaaggactttttcaataattacttatc
taaagatatggtggaccgccttgaggttgttaataaacgctttgttagagtcatatttacgccaggaaagtcacctgttgatgggcgttatgtctggttt
aatattggcagtgtggacacttttgagaggaatttggaaactgtgcagcaagagcttggtatagaaggagaaaacaggataccagttgtgtattcttctg
aaagtgatggctccttcctgctgagtatgcttcctacagtactgata
3' EST: CX971946 [+]aaattttagggtttgtatttcatcaaaattaagtgcttatctatatatgacctgcttccatttaccaatttcactataatatattccgtgcaatttggta
aaactcaaactttcagccataactggatttgcggtacatcttggcaagcatcgtacagtgaaagtgttaaaagtacaaccacaagtaatgccacaattaa
gttcttacaggaaaagacccaggtaagtgctacagtacatctacatgtacattcagtgctgctgtaatatttatataataaaaaaatgatgattcattat
aggtcctttaaaaatatgaataaaaggccatttctgtgggacctataatttgagtccaagatgctcagtctcagtcacaggtttacaagctccatataac
aagtgaaaacaaaggcttactgaccgaactgtgcctggtaagttaagatgcaatgtttttgaactccgtatcaaaaggttcccagtacccacaatgttaa
ggagttatagtacataaacatgcatagggttggataaaacgaacttaaatctaacctttggataaaatcgacaatattgcaaaataaactgccccataga
tacctttaatccttccttattccttttagacttattttaaattattttactgtgctgtatggtgtttaggctttgcagggatgggaagacatagtattct
agtatgtgccctggttgtggaaggcagaatacatatcacatcacaaaatctcaaaaaaattgtagatatctgtttaaaaacactgactggcgtagggtga
catttcataggctacattggaacatgttacagagagagcatgcagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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