XB-CLONE-3555137
Clone Name: IMAGE:7713610
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Gene:
ihh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972004 [+]
gaccgggatcgcaataaatacgggatgctggcaaggctggcagtggaagctggattcgactgggtctattatgagtccaaagcccatatccattgctcag
tgaagtcagacgcctcgttcaaagggtaccccctatccaccccccagaattccgcctggaagcccccgtcccataggaagaagctggaggaagcgcagga
tctgtgcacagactggaggaagcagggagcggcggacgcgcaggtggagctgggttatggacacatggataactatatgaggttggcagttacggatgaa
gtgggatccacaggatggaagtcgaggaagaagaaaaagaagggaagaaagaagaatgaaaaagaagggaaggggagggaggagcagaaaaagagaaaaa
ggaaaaaatcagggaaaaagcaaagtctgaacggcaggagggacggcaggaaggagagaatgggaagaacagatacagagaactgagaatggagggaggt
gggatcaggggaatagaaaggagaagggctggggggctggaccccccgaatacagcagcataaaggggaaataagccatagaaaagggggtttgatttaa
tgcttgttggacaatccccaaatacccagctgtgtagttatttattgtaattgggccgggggggcctattgccttcactgattcagttctacctgggaaa
cccacctacagacagatccacataaggaaaggtagggaccaattagaatatattgcgagtaactactagtgagagagctactaactgcactcgggcctct
gatacactcagccacacctcttccctggtctcaatgttaattccatacgtaattgctattgaaagtagtgtctgtctggctgtgtgtaa
3' EST: CX972003 [+]tttaaatagttcttacatttgtacagaggcaaaatatacaatatatgattattacggtaccaaataatataaaccagaaacctatatacactcgcaatat
attagcttctatctctatctgtaaaaatagcgatctctgtgttaacttaggcatctgcagactcatagatacgttggggaaatattcccagcatcccctg
tgacactaacactggctgagaatgctgggagttggagtttgggcggtgttgtaatgacaagctccgcccccagcacctctttgcctgtatatagggggcc
ccaaacatccctggggccccaaggcaatgccactgatcagaaggttcacattagaactagtgcagcttaatgattttatacaataattaaacattataca
gactgagtggggggctgctgcttggtcggtgccccagggagtctgcaacacaatgaacagcaggtggcgctgttgcttcaaatacattatatgaacagta
tcaaaactgccgatatctggagaaactacaaaatataattcatgtaaacaggaaaagtgcttagaaaatgatgaacgaaaaaaacgtgattctaagcaat
gtcccatagttacgggtaaatgttattggtagtgaaagcaaaatccgtgggggtcatttacaaattacaatataaacgcggacccactgtgtgtcccgtt
ctgttctctggttccgactcctgtaacaatgtagtagatcagttctcctctgttaatccgctgccttctgctgcattgtttcagcagtcagaattagcag
tgcagatattatttacacacagccagacagacactacttttcatagcaattacgtatggaattaacattgagaccagggaagaggtgtggctgagtgtat
cagaggcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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