XB-CLONE-3555111
Clone Name: IMAGE:7713660
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Gene:
slc3a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972021 [+]
acgttgaactgaatgaattggatcaagagaaggtcccaatggcaggtggggctggggacagcccaactggagggggagagaagaatggcgttgttaaggt
gaagcttgaagatgatgatgcgattggaaccaagagtcagaaatttacagggttgtctaaagaagaattactgcgagtagctggtaccccagcatgggtg
agagtccgatgggcattgctgatcctgttttggttaggctgggctggcatgctagcaggagctgtggtcatcattgtacaagctcctcgctgccagcctt
tgccagcaatggaatggtggaacaagggacccctctaccaagttggagatccagcaacctttcaagaagatggggcaggaaatattcagagtatagagaa
gcgccttgaatcacttaccagtctgaaaataaagggtttgattattggtccaatacatgctacaaagaaagatgaaattgacaaaaccatactcactgat
attgaccctaattatggcacacaggagcaattcaccagtctgctggaagctgcgcgcaaaaagagtatccagatcatcttggacctcactcctaattacc
gcagtgaaaaaagctggtttgaaaagactgacattaactttgaggataacgtaaaggaagcaattaatacttggctggaacgcggtgtcggaggtatata
ctttggagacagtgagaatttgcccaatacaagcaactttatatatgaatgggggaacatgactgccaatttcagcaaagaaggaaaaccaagagtcctg
ctgttatcctcaaatagtactcaaaacaagcttgc
3' EST: CX972020 [+]caatacacaagatccacagacattcatattcccctggctgcggcccatctctccatgctcagagggctaaaagtgccacacgcctttaaaacatgaaatg
acaacataaaaacacacctcagagcacgtgtatgacaaaactaagcagaaaacaacactccacttattgctcttaacgcgtttcagaaactgtttttaag
tttttatcctctgaacatgaaaaatatgaccgcttggcaaatataaatggcaggttacagtatatgccactgccacaaagggtagggatgtgttaaaagg
tttaaagggaaggttaacaacatgtgcttcttggcactggaaagaagggtcagcaagtggaatgtgtaagtgtttgcccctcacagggatgtagggggtt
atccactataagggtatttaaggagtaaagcctctccagctcctaactgcaagctttttagggaaacactttccccttcttttcgttgggagctggaact
caacactactgtgccgtgttctggcagctcttcgcctctgtcctttttcaaggagagttctacctctccctcatagttaaagttcaaggctgtcacatat
cgctcattctggtcccagctccttaggaaagctatggccttgtcactgttatacagtaatacaaattccccatgcaggagggatcgttctttcccacgca
ggtcactcaatttcctgtatatgtttaggaacgaccccttgtcagtatcctgtgccttanatgtgacatttgcatttacatcggataagatttgtgggga
attagcagccactgaaacttcatcccactngcatgtaggtct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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