XB-CLONE-3555032
Clone Name: IMAGE:7713524
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Gene:
nr1d1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972077 [+]
ttcagcttatatccagagaaatacgggatttgctgttccaccaatgctgcagaggctgatgcccttaaccccccaaaacccttgtgcaaggattgcttgt
tgggggtatctgctgccaggagctttgtaaccgaccccgacccaacttgctgtgtatggttctgggacccccaaacatgactacgctggattgtgctaat
aacaccggaggtgtaataagctacgtgggctccagtggctcatcgcccaatcggaccagccctgtctccatgtacagcgactgctctagtgggagccccc
agagtggtgtcagccactatgccaactacttgcctccatctccctcccactcctacagtgccagctcctctggatctgggggagaagcctcgccacgctc
gtcctacggtcttgcagcttccccgggggggcttcatgttgcactggatgatggaagcagggtgtctcctagcaaaacctccagcagcattaccaagctg
aatggtatggtccttctgtgcaaagtctgtggagatgtagcttctggatttcattatggcgtccatgcttgtgaaggttgcaagggctttttccgacgca
gcatccagcagaacatccagtacaagaaatgcctgaagaatgagacttgctctatagtgaggatcaataggaaccgctgccagcagtgccgcttccgcaa
gtgtctctccgtgggcatgtctcgagatgctgtgaggtttggtcgtatcccaaagagagagaagcagcggatgctggcagagatgcacagtgctatgaac
cacattgccagcggccattttggaaggcgaagtccat
3' EST: CX972076 [+]tttatatccaccagggttcttatttacaggtcatttgtacatatttatgtgatatttatatatctatatatatacaggtctatgtacatccctctgaaaa
tgttcccaaacaagagagccatgattcagcctttcccatctcttggaagagtctataccattgaattactgtctatgaggtcaacggcacaaagctgatg
tcatacttcatcatcgtcatgtggttcatccatgttttagcagcttcctgtgatccccctgccaccttatcactatgattccacctccgtccgtcctgcc
cgtgatcggccatatctccaaataggcacagcttgtatagccactcggctttctaggttcagtttaaagtgccagtgctctgcccaaccaacgcttaggc
tccatggtgccatcagtgggcatcgacacgaaaggagagcagtttctcggagtgcatgttgttcagggtgcgcaggtccgggaggcgcaggagcagcttg
gtgaagcgggaggtatcgtttggactgttcttcaggatgagggaacgaagggcacgtatgagagtctcttgaagctgctctactaaggaggaattctcca
tcccagagcgatctgcagacaccagtacaagtgctgtaaagatgcccagctcctcctggctcagactgagagatgacagcttttcactgaagtcgaacat
ggagctgagaagttcacccattcccatagcatgcagttcaggaagggagtaagtggcgccgctgaggaacctcaaggaatgttcccgagcatcaaacaag
gaggcaaagcgcaccatcagtacctcaaatgtgccagccttgagcagggtgacct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: