XB-CLONE-3555017
Clone Name: IMAGE:7713382
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Gene:
ybx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972087 [+]
ggaagttaaagcgagccaaccgccggcccgagtcaccacctccgaaagcagccaacttacaccatcaaagacacaatgagcagcgaggttgaaacacaac
agcagcagacagtcgcattggagggcaaggccggccaggaaccggcggccaccgtgggggagaagaaggtcatcgcaaccaaggttttgggtacggtcaa
atggtttaatgtgcgcaatggttacggcttcattaacaggaatgacaccaaggaagatgtgtttgtacaccaaactgccatcaagaagaataaccctagg
aagtaccttcgcagtgtgggagatggtgaaactgttgagtttgatgtagtggagggtgaaaagggtgcagaggcagctaatgtaactggtccagggggtg
ttccagtccaaggcagcaaatatgcagcagaccgtaatcatttcaggcgctatcagcgtcgcagaggtcctccacgtaactaccagcaaaattaccaaaa
taatgaaagtggagagaagacagaagggaatgagagtgcacctgaaggagaggggggttcaaatcagcaacgtccataccgcaggagacgtgttccacca
tactacttgcggagaccatatgggcgcagaccacaatactcaaatgctcctattcagggagaaggtgcagagggatcagatggtcaaggttcttcacaag
gtgatcaaggtagacctgtgcgacagaatatgtacagaggcttcagaccacgattt
3' EST: CX972086 [+]cttttatgttgagacctttattaacagatgcttgcagtttgttgactcttgaaaaaaattaggtgtacacttattacaaattaaaaatgaggttcttaaa
aatcttagtttgaccagataggaaacaatttaaaaacctttgaaaggtacattgagaaaaaatatgtttttaaaaaattttcattcccaaaaggagcttt
tagaaaaaaaaacataaaaaccatctctgcatttacccatgcaggatgaagttatctggtcaagagcaaaaagcaagcacttaaggtcttcagttccaat
tctttgttcatttcttattgctggaatttctgtcttgacattttgatgacaaaaaaccggatggtggtagagttggtaagccggtatttactcagccccg
ccctgctcagcctcgggagcggacgtgttctcagctgatgtttcggctgccttggtctctttaccatcttgtggtttagggttttctgggcgtctgcgtc
tgtagttaaagttacggcgataccgacgttgaggtgcctgctgactctgggtttcatccccctgattttctttgtcctcttcatttccttcttctctggg
ttgtctctgccgtggtggtcccctgcgaaatcgtggtctgaagcctctgtacatattctgtcgcacaggtctaccttgatcaccttgtgaagaaccttga
ccatctgatccctctgcaccttctccctgaataggagcatttgagtattgtggtctgcgcccatatggtctccgcaagtagtatggtggaacacgtctcc
tgcggtatggacgttgctgatttgaacccccctctccttcaggtgcactctcattcccttctgtcttctct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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