XB-CLONE-3554989
Clone Name: IMAGE:7713480
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Gene:
bmi1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157200 [+]
AGAGCGCTCTCTGTACTGACAGCGTCTGCAGCTCACATCAAGATGGCCGCCCCTGGGCTGCTGCTGCTCATCCGCTGAGCGCCCCCCGGGCACCGTATTG
TCCCCGGACCCCCCAGCAACAGCACCCGAACGAGACCCCACTTCCACCGCAAAAATGCATCGGACCACCAGGATTAAAATTACCGAGTTAAACCCACACC
TCATGTGTGTGCTGTGTGGCGGATACTTCATAGATGCTACAACCATAATCGAATGTCTCCATTCCTTTTGTAAAACCTGCATCGTTCGGTATCTGGAGAC
CAGCAAATACTGTCCGATCTGTGATGTTCAAGTACATAAAACCAGGCCCCTTCTCAACATCAGGGCAGACAAAACCCTTCAGGATATTGTGTATAAGTTG
GTGCCAGGGCTTTTCAAAGGTGAAATGAAGAGGAGAAGAGACTTTTATGCTGCTCATCCTTCTGCAGATGCTTCCAATGGCTCCAACGAGGATAGAGGAG
AGGTCGCAGACGAGGACAAGAGGATCATTACGGACGATGAGATCATTAGCTTGTCCATCGAGTTCTTTGACCAAAACAGGGCTGATCGGAAGGGAAGCAA
AGACAAAGAGAAATCCAAGGAAGAGGCCAATGACAAGCGCTACCTGCGCTGCCCAGCCGCCCTCACAGTCATGCATCTAAGGAAGTTCCTGAGAAGTAAG
ATGGACATCCCCAGCAATTTCCAGATCGATGTGATGTACGAGGAGGAACCGCTGAAGGACTATTACACCTTAATGGATATTGCTTATATCTACACCTGGA
GAAGGAACGGGCCCCTTCCGTTGAAGTACAGAGTAAGACCGACCTGTAAAAGAATGAAGATAAACCCACACACCGACAGGATAACCAACACTAGCGGGGA
CATGGAAAGTGACTCTGGCAGCGACAAAGCGGGCAGCCTGGGTGGCGGCATGCCTTCCACCTCCTCCTGTATGCCAAGCCCCAGCACCCCTGTGCAGTCC
CCGCACCCACAGTTCCCCCACATCTCCAGCACCATGAATGGAACCAGCTCCAGCAGCAGCCACCAGAACCCCTTCGCCAACAGAGCAAGGAAAATATCCT
TAAACGGAGCCTCTGCCATCTCTTCGGGATGAGGGCGCCCCCTAGGGATGGGAGGACTGGCGCACCGCCTGGTTCCTAGATCTCTCCATACCATTGCAGC
TTTCTAGATGCTAATATGTGTGGCGTTTTGTCCAAATCGCTTTCTTTGTAGTGAAACCATATATCGCCCAGGAGTGAATCCAGAGGATTATTATTTAAAG
GTTACAGTGAAAGACTCTTGAAAGTGTTATTTAGTTGGCTAACCAATGGTATGGGATGTGGATGTCGCGCTGGATGCAGGGGAGGATACTTTTGGATGGA
TGAGGCATTGGCTTTATTGACCTTGGAACAGGGCATTGTTGGGCCACTGTGGATATACAAACCGTAGCTACACTATGTGGGTGGCAGTTAGGAATGATCA
TGTTGGATGTACAAGTTGTGCACTTCCAGTGGATACCCTTGGCATCGCATGGATCTCACCCTGCTGGATATATTGTGTGCCCTCTTTGATAGCTCTGCTT
GTTGTGTTTGGGAAGGATCCAAGACCAGACAAACGGTGGATTAAGTCATTTGGAGTACAAGGGGGATCTCTTGGGAACAGACCCACCACCAGCTCTTTTC
TGTGTGTGGACTAATAGAAAGCGATCATCTTGTCACATCCAGCCGAGAGATACATAGAGACGCATCTATGTAGCCATTTCACTGTGAATAGCGTTAGCCA
TGTTGATTTCTTTCTTTTTTTTTCATATTTCATTTGTCCTGGCACCCAATTTACTACCAGAGCACCCAGCACCCCAGATGGCATATCTGGAAGCAAATTG
GTTCATTTGTCGCTGCACAGAATTGGTCAAGTTATGAAAAAAAAATCATGAAACAAAAAAGAAACGCATTGTTTTCCAGTTTTACAATCTTTGCCTAAAG
AAAGCGGGTATTACCGTTTTATTTTAGTGACTTGTATAAATGATTCGCTTTTGTAAGAATCAGTTGGCATTCTGCGTGTTGAACAATGCCATATTGGTAT
ATGACATAACAGGAGACAGTATTGTATGATATATTTATAAATACTATAATGAAAATTATTGTGTTTCATGCACTCACAAATGATTGTTAAGGTTCATTCT
TTTTTTAATTATTTTTCCCAAAAATCTATCCGAATTGTCAGTTTGATAGCACAGCCTTGAGGAGTCCCAGGGGCACAAGTCTGTTTGTCAGCATCAAGTC
AGTATGGTTCCAAGCAATAAGGGCCCACAGTGTGTTTGTTTTTAACTCTCCTAAGTACAGTGTAAAATCCACAACTTGTTTGGGAAGTACCAGCTGTTTG
CAGTGTTAAATTAAAACTAATAATATTGCTATTATCTGGTAAAATGACCCCCCCCCCCCTTCAGTTAATAATGAGTTTCTTACATCTCTTCAAGGACCAT
TTTCATAATTATCTGAAAGGTTTTTGATTATTGCCCCCACACAGCTGTGACAGAACTACATCTCCTAATCTACCTCCATAGCCAATTAGTCAGGGGGTAC
TAGGGGTTGTAGTTTAGCCACCAGTAGATCTGCTCTGATCAAGCTCCTTGCATCTTCAATATATTGGCCCAGAGAGTTGACAATAGACTGGCTTGGATCC
AGCCTGGTGTCTGCTGATCATTGAGTGTAATGTTGGTAAGGAAATTCGTGGCACATCATTTCATTGTCTCCAGTCTACAAATGAAAGAAGCACAATTCTA
TTGCTTTCTCTTCTCGCTTATAGTCATTAAACCGTTACTTTTACAAAAAAAAAAAAAAAAAA
5' EST: CX972105 [+]agagcgctctctgtactgacagcgtctgcagctcacatcaagatggccgcccctgggctgctgctgctcatccgctgagcgccccccgggcaccgtattg
tccccggaccccccagcaacagcacccgaacgagaccccacttccaccgcaaaaatgcatcggaccaccaggattaaaattaccgagttaaacccacacc
tcatgtgtgtgctgtgtggcggatacttcatagatgctacaaccataatcgaatgtctccattccttttgtaaaacctgcatcgttcggtatctggagac
cagcaaatactgtccgatctgtgatgttcaagtacataaaaccaggccccttctcaacatcagggcagacaaaacccttcaggatattgtgtataagttg
gtgccagggcttttcaaaggtgaaatgaagaggagaagagacttttatgctgctcatccttctgcagatgcttccaatggctccaacgaggatagaggag
aggtcgcagacgaggacaagaggatcattacggacgatgagatcattagcttgtccatcgagttctttgaccaaaacagggctgatcggaagggaagcaa
agacaaagagaaatccaaggaagaggccaatgacaagcgctacctgcgctgcccagccgccctcacagtcatgcatctaaggaagttcctgagaagtaag
atggacatccccagcaatttccagatcgatgtgatgtacgaggaggaaccgctgaaggactattacaccttaatggatattgcttatatctacacctgga
gaaggaacgggccccttccgttgaagtacagagtaagaccgacctgtaaaag
3' EST: CX972104 [+]gtaaaagtaacggtttaatgactataagcgagaagagaaagcaatagaattgtgcttctttcatttgtagactggagacaatgaaatgatgtgccacgaa
tttccttaccaacattacactcaatgatcagcagacaccaggctggatccaagccagtctattgtcaactctctgggccaatatattgaagatgcaagga
gcttgatcagagcagatctactggtggctaaactacaacccctagtaccccctgactaattggctatggaggtagattaggagatgtagttctgtcacag
ctgtgtgggggcaataatcaaaaacctttcagataattatgaaaatggtccttgaagagatgtaagaaactcattattaactgaaggggggggggggtca
ttttaccagataatagcaatattattagttttaatttaacactgcaaacagctggtacttcccaaacaagttgtggattttacactgtacttaggagagt
taaaaacaaacacactgtgggcccttattgcttggaaccatactgacttgatgctgacaaacagacttgtgcccctgggactcctcaaggctgtgctatc
aaactgacaattcggatagatttttgggaaaaataattaaaaaaagaatgaaccttaacaatcatttgtgagtgcatgaaacacaataattttcattata
gtatttataaatatatcatacaatactgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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