XB-CLONE-3554969
Clone Name: IMAGE:7713482
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Gene:
eps8l3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972118 [+]
aaagtggatggacttctggcattcaaggacgtaatttttagaccaagtaagaagcatttttcatattgaagatggataattacatggatggaattgatgg
tgtactggcacagcgtgaaggcttgaccagatccagaagcataagtagaccaactgccaagaatatctaccaacaaaggaaacagtacgcccaatcatta
tctacagcggtcaccaactttcagcacagagtagagcacctcttgacttgtgacttggacaaagaacttcgcaatgttgaggactgtttgaaacatctta
gtttccttgaggcacagggaagaatatggggtcaaggactgattctacaattggaaaatggagaaatattactgaaagacccagaaaccaaggacagtct
agagcaattacctctgcaaaatgttgataactgcagttctgtgttggggggctctacatacaattcccttctcaccattaaagttcagactcaacagaaa
acaagcatcttcttcttccaatgtgatgaacagccggtgagtgaggtcactacaataatataacagaagatagagaacatatgttttactcaattttttt
tatcttcctctaccatttaggctgatgttcttcatgcaaacctagaaaaggcattaaagcaattcagattaaacaaagaaagcaaggatacctacaggtg
ttttgtaaccaccatatttttattataaagcactgtggaatatgttggtgcattaaaaacacttataatagctatattatgtatgctgtgtaggttccgt
gacagttaatatatctttttcctcttaggtataacttggagaactct
3' EST: CX972117 [+]tttgatcttagtcaccaaggcaaaagttcaaaactttaattctgaagtagagaaactgatctgcctttttaaaaggtataattttatttgaactgatgtg
ttgcaaatactggttccaaaaaaataggaaaaattcaaactaaaattaatcttggaatcctgttatattatattgagttatattgaggttttctttttaa
tttctgaatggtttgacttgttattcttcagttttcattctggaaggcttgcaagggtcagaaaccaaataattcccccagtccttttcttcatagtacc
tgaccatcatctgtgttctccaggatgttgcttggaataaatccctgctggccctgagaattctgcaccatccaccactgccggctctggtccaaaacct
tcacaatgtctcctttccttactgacagttcgcgattatttctcgcttcaaagtcatgaaggacattcatgaggagtgggtgaaattgcctgtttcgtag
tggtgtttcaggactacgtgacatctgggatgaaacatcactaaaggggatatcagggatgatccacccatcagaaaaagttggaacatattgaggtata
ttctttccatttggccattcagctctggtcttcatccaggcatctcccaggctttcccagaattttctttctttgtctgtcacacaagaactcataatca
tcagtgccttctgtgtaagcagtggagataacacagactgtggcaagtctttttgtggacacctgtctacaatcttggccagggtttccatcatcaaatg
gatgagatctggagcacttggttgtttcatttgttcgtggacattccccatt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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