XB-CLONE-3554916
Clone Name: IMAGE:7714013
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Gene:
cldn3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972156 [+]
aaaaggtgttttaatatatacataataatgataaacttgatgtacaatgtacaatgcaatgcaccatcactggcgtaaatacaatgtgctcctagatgat
gggacagaaaacatgtacatttcatatctacctgcttactaactgatacacaatattaaagaaaaacaaaaggttaatataaaacagtggtagcaatgga
aggaatacaaatcacacaaattttggtgcagagtaaaaaatgctgttgaaattttcatccaaactgcaaaatgcttttgtgatgtgttgattagttgttg
aagttaaaatatgaaacagaatttcactgggataacaaaaaggtgtggacctatacatttatacatgtgtgtgtagagtatatataaacatttaactgcc
tatctatatatttatatgcatatttctgtacagtgaatgtgcctccattgataaaatacaaaagaagttaaaataaaattgtatctctggcgtaggtagc
ttgcaacactccatctgtttcatgttccttagctaggacagcctagttagaggccatgggactaagaacaggtcttgtgagtgacccacttgtgtgaaat
aagtgactgtttaattagagttttttactgcttgatgctcattagaaagacctcattgactgagatactgaaattatatccagggacatggtaattaaaa
taaacattaaaaataattgtagatagtaaggtttagaacttatttttgatgaagtgtattcttgcgtataaggggcagagttaacattccagtgccatat
ctaacaccataggtactgctagtgagaagtgctatatctgggctgttatccattaac
3' EST: CX972155 [+]ttctggccttatgacacttatccctgtgtcctggtctgcaaacactattatccgtgatttctacaatcctttggtagtggatgcacaaaagagagagctt
ggctcctcaatgtacattggatgggcagcctctgctcttcttatgcttgggggagcaatgctctgctgttcttgccccccaaaagagaagtaccctactt
caagggtggcttattcagctgccaggtcaacaaatcctggttatgacagaaaagactatgtctgaaatggacattctgggtccttatgaaggactgaagg
gactgtcttgtattggacttgtgacaagcaaaagggagcaaaaggaagaagttaccccctccataaactaacatttacaattattcctctaggtactgca
tttgttgatatttgttatatatatgctatatattattttagatgtattgccccttttgactgcacagtgtgcatttgttgtgttagtgtttgaatgtgca
ctgtaaagatattaatgttttccattgccgtggcatttgtagaatattcttgaacagggaggggcattcggtggcagagatgaggtggctgtactgcatt
aaccgtttccataaatgcagcttactttcttttgtctaaatacaaagcattacactgctctatccattgatatttcattatccatggaaacctcccatta
gatactgatgcaataccattcccaaattaggcaatattattgattactgcaagatgatatatttaattattctcatggatgaattttgcatttggacctg
ttttgttgctagtagaatctgatggtctatactttgtttccaacatagagataatgcatcagtttttacag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: