XB-CLONE-3554878
Clone Name: IMAGE:7713873
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Gene:
gpd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972181 [+]
gagaatacacctgatattacacacagcacgcaccagataaaacccccttttaatataatacatctatttttcttcaatcgtttgtcccaattatgtgctt
gatgtgcacaaggggcaaatgttcccctctaagatcaccaattgaaaatgcgtaattactatcatttcccactgcttatttacaaagatatgcccgggcc
attaaaatataataatcattaggctaaaagcatttaattgcacattactaagcatattctcccatcagagtttccttaaaaagctctgcagaagccctgg
gtgaaggcgccactgtgtaaggaaaactcagcatctgctttaaaatcgaaataaaaacctgctatgaatttgcctgaacaaatatcccatcaagtaaagg
tttatgtagcacccaatgccacctctctctgccactggcaccgggctaacccataaggctgccgcacagtagttatttcacatacaaactaaactatatc
agggttaagcaaccactaaccctgctggtaggatggattctgggaattgtagttccacagccagcacaccagcagatgcctgccccagctctataaatat
ataataaaatatactcatagaaaaccccatttctaagtttaaaataaggcacaaggttgaaggacaaggattgccagggctgttccactttacatgtgct
cggggtggttctgcagacacgttatgaattctgttactggctttccctcgtagcagatctgatagactgccgtgaagagcgggaactttcccaccaagtt
cttgtttttcagaatgtgatttagttctgccgaggtctgtggtccctgtagcttctgtcccgtcagcatctcttgttccagctgttctatagacttttcc
ggtc
3' EST: CX972180 [+]aattgggggggaagaagctcacagaaatcattaactcagaccatgaaaatgtcaaatacctccccggacacaagcttccagccaacgtggtggctgtgcc
tgacttacttgaagcatctgcaggagcagatattctgatctttgttgttcctcatcagtttatcggcaagttatgtgaccagctaaaggcacatgtgaaa
aaagaggcctttggcatgtctctaataaagggtgttgatgagggaccagagggtctgcgtcttatttcagacatcattcaggagaggctaggaatccaga
tgagtgttctaatgggtgccaacattgccaatgaagtggctgatgggaaattttgcgagacgacaattggctgtaaaaataaagagcatggcaaaattct
gaaggaattgttccaaacccctaatttccgtatcacagtggtggaagagaaggacacagtggaaatctgtggggcactgaagaatattgttgctgttgga
gctggcttctgtgatgggcttggatttggggataacacgaaagcggccgtaatccgccttgggctgatggagatgattgccttcgccaagcttttctgct
caggatcagtcacgtctgccactttcctggagagctgcggcgtcgctgacctcatcactacatgctatggcgggaggaacaggaaagttggtgaagcttt
tgctcggaccgganagtctatagaacagctggaacaagagatgctgaacggacagaagctacagggaccacagacctcggcagaactaaatcacattctg
aaaaacaagaacttggtgggaaagttcccgctcttcacggcagtctatcagatctgctacgagggaaagccagtaacagaattcataacgtgtctgcaga
accaccccgagcacatgtaaagtggaacagccct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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