XB-CLONE-3554875
Clone Name: IMAGE:7713921
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Gene:
calr
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972183 [+]
aggaggaaaaagaggaagaaaaggaagaagaggaggaagatgaagaagaaacacctctaaaagatgagctttaaaaccatggcagcttgtggctgtcgcc
cctatctggcgctctcccctaccctgggctttcatgtcttagataggagaggggaaatgggatcgttctgtctctatgagactcaaatttatatatattt
tttactgtttttgttttaaccctttaataggaagatggtcagtcaatcttccgtcccctttcccccatctcctctctcctccaccttttggtttgtccct
tcaattctccaatctcgtctcttggtcccttcccttcagtccctccattgcagtaactctttgccttcctcaattaggaaataaccacgcccacagatat
ggtctggccaatcaaaatcatgccagatttgtctacgttctgcctgggaatacccagctcaacaagggttccttaggcagggcgacagttgtatttgagt
ttcctcccgtaatgcagtatcgaatcaaatcagtgttcatattttaaaagccaagcattgataacatggccgcttgcgtgttaaaagcccagctagtgtt
ctctatgtggggctattgcctggtggggaagtggtggttttaataagccgtacagtgctggccagtgtggccctcggctggctagcatagaactcaagct
tagcatacacacagccatacttctctgcacctataccgggggcctggattagaataggggggttggctgctagtgaccctgcggcggtaacacaattgtg
catgggtgaaacagggctattgctattatcattagtgattgcttacagcagtaaatcccagcctgcgg
3' EST: CX972182 [+]ggacacaaacatatttaattagaaatctgacatctgtacattccaaaacaaaacccatcgaggctccagggatgagccctgccacaatataaattagagg
cgtaaatcaaactggggattggaggacagtggtctttataaaaggaatgaatccccctggacactaaaaagggaatacactggcaggttggttattggac
aacacttgtggaatgcagatggaggggccgcagccaatctgtataataccctgcaggtttagcaaagtcacctaacactataaggcattgctgtagagct
gggagtggtagttcagcaggccgcaggctgggatttactgctgtaagcaatcactaatgataatagcaatagccctgtttcacccatgcacaattgtgtt
accgccgcagggtcactagcagccaacccccctattctaatccaggcccccggtataggtgcagagaagtatggctgtgtgtatgctaagcttgagttct
atgctagccagccgagggccacactggccagcactgtacggcttattaaaaccaccacttccccaccaggcaatagccccacatagagaacactagctgg
gcttttaacacgcaagcggccatgttatcaatgcttggcttttaaaatatgaacactgatttgattcgatactgcattacgggaggaaactcanatacaa
ctgtcgccctgcctaaggaacccttgttgagctgggtattcccaggcagaacgtagacaaatctggcatgattttgattggccagaccatatctgtgggc
gtggttatttcctaattgaggaaggcaaagaagtactgncatggagggactgaaggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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