XB-CLONE-3554869
Clone Name: IMAGE:7714017
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Gene:
hpse
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC160516 [+]
taaaagaggaagctgaggttggccactgtcctattttgacatcagcatgtaattatgtgcaactcttgcaaaaagtgaagctgctaagaacagggtacaa
ttgaaacagatttctttgtttttcagaatgctccagaagaaactgcttcctggtttcggcttgtttgttttaatagattcctgccgtggcaggctatcag
tcttacattcatattccttggtcctgtgtaagtaataaattgagcaatgcatccctctatgtacttagtttttctggctgctgcattagtgagggctgcc
agtaactctgggaatgccactgtggagctaacactggatctgaagacaaagattaaatcctcaataaacccacgattcctttctgtaactgttgatggca
gccttgcctcggaacctaagtacattgctttccttaggtcacagaaattaatgaccttagccagaggtttatcacctgcttatcttaggtttggaggaac
aaagtcagattttcttttgtttgatccttcagaaaatgaatcccaggaacatcttacaacaaatcaagctgtcaaattatgtgatcaaaggaccctttca
gctgctgaagaaaagatgctacgttctcattggctgttacaggaacaacgaatactcaaggagaatttcaggaatcagtatacaaatgccacaattacaa
agagcactgtggacatgctgtacaggtttgcaaactgttcaggtttacacctgatatttggacttaatgctttgctgaggcaagataacaatgaatggaa
caactccaatgccaaactgcttattgattactgttccttgaagaaatataatttagcatgggaattaggcaatgaacccaacagcttcaggaaaaagtct
ggaatttatattagtggctcccagcttggcaaggattttgtgaacttacacaaccttctcagcaattatccaagctatcgggactctgggctgtttggac
ctgatattggccaacccaaaacacagtcacagaagatgctcaaaagtttcttagaaactggaggtgatatcatcaattcaattacttggcatcactatta
tgtcagtggtcaaactgcatctgaggaggattttatcagtgcagatatactggacacacttgtctcggagatacagacaatattccagatagtaaatgag
agtgtgccagggaagcacgtatggttgggtgaaacaagttcagcctatggagggggatcccctggattatctaatacataccttgatggcttcatgtggt
tggataaactaggaatcgcagcaaaatcgggaattgatgtagtgatgaggcaagcactctttggcgcaggatcctataatttggtggatttaaattttga
acccttaccagattactggttgggtttgttatttaagaagcttgttggatccactgtactaaatgccagtttcaaaggcaccagtaggagtgacaggaag
cttcgtgtttatatccattgcacaaacaacaataactctaaatatgttgcaggagatgttaccctttttgctttgaacctgcatgacaccacaaagaaca
tgcagttaccgagttccctatcaggaaaatttattgatgaatatctgcttttacctgctgggaaagaagggctacattctgcaactgtgctcctgaatgg
cgaggttctgaaaatggttgatgataaaacactgccaactttaaatggaaggctactggttcctggcactacccttgtcttaccccctctcagttttgcc
ttctatgtagtaagaagtgctaaggcaagtgcctgcctgtaagacctggaaaagatttttatactggaacattatttcagtacagattaaattattttat
ggaccagcaaaaaaaaaaaaaaaaaa
5' EST: CX972187 [+]taaaagaggaagctgaggttggccactgtcctattttgacatcagcatgtaattatgtgcaactcttgcaaaaagtgaagctgctaagaacagggtacaa
ttgaaacagatttctttgtttttcagaatgctccagaagaaactgcttcctggtttcggcttgtttgttttaatagattcctgccgtggcaggctatcag
tcttacattcatattccttggtcctgtgtaagtaataaattgagcaatgcatccctctatgtacttagtttttctggctgctgcattagtgagggctgcc
agtaactctgggaatgccactgtggagctaacactggatctgaagacaaagattaaatcctcaataaacccacgattcctttctgtaactgttgatggca
gccttgcctcggaacctaagtacattgctttccttaggtcacagaaattaatgaccttagccagaggtttatcacctgcttatcttaggtttggaggaac
aaagtcagattttcttttgtttgatccttcagaaaatgaatcccaggaacatcttacaacaaatcaagctgtcaaattatgtgatcaaaggaccctttca
gctgctgaagaaaagatgctacgttctcattggctgttacaggaacaacgaatactcaaggagaatttcaggaatcagtatacaaatgccacaattacaa
agagcactgtggacatgctgtacaggtttgcaaactgttcaggtttacacctgatatttggacttaatgctttgctgaggcaagataacaatgaatggaa
caactccnatgccaaactgcttattgattactgttccttgnagaaatataatttagcatgg
3' EST: CX972186 [+]gctggtccataaaataatttaatctgtactgaaataatgttccagtataaaaatcttttccaggtcttacaggcaggcacttgccttagcacttcttact
acatagaaggcaaaactgagagggggtaagacaagggtagtgccaggaaccagtagccttccatttaaagttggcagtgttttatcatcaaccattttca
gaacctcgccattcaggagcacagttgcagaatgtagcccttctttcccagcaggtaaaagcagatattcatcaataaattttcctgatagggaactcgg
taactgcatgttctttgtggtgtcatgcaggttcaaagcaaaaagggtaacatctcctgcaacatatttagagttattgttgtttgtgcaatggatataa
acacgaagcttcctgtcactcctactggtgcctttgaaactggcatttagtacagtggatccaacaagcttcttaaataacaaacccaaccagtaatctg
gtaagggttcaaaatttaaatccaccaaattataggatcctgcgccaaagagtgcttgcctcatcactacatcaattcccgattttgctgcgattcctag
tttatccaaccacatgaagccatcaaggtatgtattagataatccaggggatccccctccataggctgaacttgtttcacccaaccatacgtgcttccct
ggcacactctcatttactatctggaatattgtctgtatctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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