XB-CLONE-3554815
Clone Name: IMAGE:7713783
|
Gene:
slc13a2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX972223 [+]
taatgatgtatttgcattagtaacatgtagctcattgagctctgaaacctagggcacctagatatttcacccaatactgatagagccattaaaatgttta
cattatttttcttaaataaatacaagtgagatacttgccctgatacaaaaaattaagttcttagagagaactttttacactcttgcctgctgggtattgc
ccttattaacagtactattaactgataaggtccccaattaaaagctttgcagagcagagtgagatgctaccttgactattctgtaggccggtagggtact
cggcccatctgcttatagtcacagggtgggcacggcttgtgcgtattcacggacactagagagtctgaactgtcaatctgtggattctggcagaagtcag
tggggctgctgtaagatgccataaagaatcactgtttattgggctgatgggggactgtttgagtctcttggtagttgaaatgccagggtctattttgaat
tccagtctgaacctgtgccacatccaccactttatcatacttctgaggctccttggcatccagttaaacacaactggcagcataaaagccaaggaggccg
atagtgtgcatggaagcatgatatacagtgggttcagatggattgctttggccatggatgctaggatgggtaagaatagggttgtggtcgccacattgct
agtgcactcagtgaatgtagccacgagtaggcacagaattagagcaatagctgcagggggtatgctttgcagaggggtcagcttttctccaagccataat
gacaaacctgattcctcactgcccttcgc
3' EST: CX972222 [+]tttggtccctctctttcttctgcccctgcccctggtagtgcccaccaaggaggcaagctgcggctttgtgatcattgtgatggcgttattctggtgcaca
gaagctcttcctctggccgtcactgcgctttttccagtcttgctttttccaatgatgggcatcatggattccacagcggtgtgcagccagtatctgaaag
acacgaacatgctgttcattggcgggctgctggttgcgatatcggtggagaaatggaatttgcataaacgcattgccctaagagtgctgctgattgttgg
agttaaacctgccctcctgcttcttggattcatggtggtcacagccttcctttccatgtggatcagtaacacagccacaacggccatgatgatacctatt
gcacaggctgtgctgcagcagcttcacacctcggaagagagagtggacaagccgtcagaagaaacaaaaaagaatgtgaatggaatggataatagcatgt
atgaatcagtattgccaaatgggaaaatgatcctggcagtagaaaacacttatgctactgtaaacgagggatttgagatgcaagatagtactaaagatcc
tgagccttccaagctagaaaaagaaagtattggcccaatagtgatagaacttgaggatgaaaaacaaaaggaagagaagcagaaacttagattggagaag
cacctgaagatctgcaaaggaatgacccttagtgtctgttattctgccagcattggtggaattgcaacattaactggcactaccccaaacttgtaatgaa
aggacaaatggacgagctctttccagagaacaataatgtcatcaattttgcctcctggtttggttttgcatttcctacaatgtttgtacttctggcatta
tcctggttatggctccag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: