XB-CLONE-3554809
Clone Name: IMAGE:7713879
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Gene:
XB5734592
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972227 [+]
ataacgtcaacatttctccctcctccttgtccattacaccatttagggaagaacatgaacatgagatggagttacaaggtctttggagatgatcactaac
aggtatcagacaagacgagggacacattcatgttagtaggtttaccagaagacttgtcaatgctcctttgggtgagctgtagtggcaaagactcatggcc
aaccacgcaggaaaaactatcaccatccttccagtttctactgtctatggtgaaaagactgtagagaaagaaggtctttcttccattagaagagagttcc
tcattgatgttggtatttgtgtaatggttcttggaaacctcctcatttttgtgaacccattttacaaatatgtcggaggggctgaaccctttaaccaagc
atgtcaaggtggcagtttcacctagagacaattcctcttgtgggggtgggaaaacatatacagatggtgaaattaaaggtgctcctttttctttagagag
agagacctcctttactgaagccagctctgtgtgttctactttgcagacaaaactgtccttattccactcatcagcacagacagttgcggttccttcttta
gaataagtgcggttatcatgatagatgtcatttcctagtgttgttttcaattgttcttctttattcccagatattttaaaccatgatattgatctcaggt
cctcagaattatccatattactaaccaggcaggtaagtgtggcagattttttctcaaaaatgctctctaatgatggtggaatagtgattactttgatatt
agtttgtggtgtttgggtgtcgcacataagtgatttgctcatatt
3' EST: CX972226 [+]ttggaccaacctggctctgcagttgtgaatccatcaggaaccttaaaactttcctgtaaagtctcagtgtctgtaagtagtcagaagtgggcatggatct
ggcaggctcctgggaaaggactggtgtatatggggtatttgagcaacaacgggggctctaacccaaccacctcatttcaaagtcgagtcactttcaccag
ggacacctcaaagaatgaagtttacttacaaatgaccagtatgaaaagtgaagactctgggacatattactgtgcagagcggaactatgctttcgattac
tggggccctggaacaatggtcaccgtcacatcagctgttacatcgcccccatccctttttccgctcatttcttgtggggagtctatggacccagtcaccc
ttggttgtttggccaaaggtttcctccctgacagtataagcttcagctgggaagataagtccaataccagtatctccaatggcgtcaagagctatagacc
tgtgatgcaagcttccggcacctactcagccagctcccaaatcaacgttgattctgcaacatgggataaaagcaaaccattttactgtaatgctaaacac
ctggaagtcaccaagcgtgtggagatcggaaaaatagactctttaagaacacagccagttgtaactattcaccctccatcaaaggatgcccttgctttga
atgaaagtctctatgttgtgtgtcttgcaactggttttaatcctaaaagtatatcagttaaatggctgcagaatggcaagcagacaacagatggtgtcac
agttgaagagcccactgtagacaaaaatggaggatatgagacaacatcctatatctccataactagaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: