XB-CLONE-3554785
Clone Name: IMAGE:7713881
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Gene:
c3h15orf40
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972243 [+]
tttacatgctactaaatctgtctttgaaagtagcaatttaaaaaatattagcattcagtctcgaaacagcactgcagtaacaaaaagctttacacgtgaa
tacagaactgaactactaaccgtatcttcatctcaaaatataaaatccattgatataaatatttttccaactatgggcccattacagaccaaccctacac
tgtttaaaagtgcaacagatcaaagtatcatgatgacaaatcctagctatccatcccataccagcaacatatcagttccagacaccttatctaatactgc
cccaacacaacctattagttcttcatcgaaattgaacacttcaccaacgaccatgacttctcccggaacaacatctgatgtcggcatttcacctactgct
agtcctgcatctgattttacaataagcatcagccctatacatgaatttgtccgtacaccatctaaaagttttcctactacacccagcttgccaacaacct
tatctaaaatggtcagtcaacaaactagagtcctacctaaaaccaccacttcacaaattactaaatctgaaagcagccatccaggaaaaaacagtgctac
accagctgcaagccttccagctgatatcacattagatgctgatcttccaactcaaaccagcattatatcagctaaacacactaaatacaaatttactgct
tctgaaaatattcctaanaccacattatcaacaagaacatctctgtttgtaaccagagtattaacaaatgctagccccctcacaaaagagaacattgcat
taacttcatctaagggtaacattctgtcatctaccagcttattgcttacacctccagaactggttgcaccaattcaaagagatcattttcag
3' EST: CX972242 [+]agcaaaatatatatacatttgcgaaagagttctgtaattgctcatggcggtgcgttttaaaggagaaagaaaggtaaaagctaagtaagctttatcagaa
aggtctatgtaaatacagccataagcactcacagaaattctgcactggcttctctgtgaaaagatttcttgtgtctgtaattcattcgccagagacaagc
tgatctctgctgtctcctctctcctggtccccccttcctcaagaatgctaagaactcactcccccccccttaggaatgtggatcctgaagtttgaatccc
gaattgggaaaaattcggattaaatacgatatttctgatgattgtaaacttaacgaaaatgtttatgaaaaaaacgttttagtcatgatattgtattggc
gatccgaaagtcacaacattttcgtatccgaacgatcataaacagcaggaaaacttttccgactttgatccatctgtgcatgattttggaagcctcccat
agtactcaatggcagtctgcagctccatcctggcccaaggaaagtcacgatatcgaagattaaatgaatccgaaactttcgtataaaatatgattttgcc
acacaaattttgtggcaaagtacgaaaaagttgcgcaaaatacgtagaagtcgcagaaaatacacacagtacgatcaaatacaatttttttgtaattcgt
acctgtcgtactttaataaatagaccttaaaatgtccaatatattttttttcgctttgctttattttggggttgtgtttcggttgagatcattgccttgt
gtcatcaaagctgggcataaatgtgggtatattatccccgacacaaggaactctcgaccctgcttctggaaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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