XB-CLONE-3554764
Clone Name: IMAGE:7713835
|
Gene:
gmds
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX972257 [+]
agaaggagcagcagtgcacagacttggcactaccggagccagcagggtacagacaccgagctatggagctcacgcctagcctcaccctgtacaagcccac
cgccttcatggaggagagccctggctatcctgccagagacttttacagctttcagaggccctggcaccccatggccggataaaggtggagagccccatgg
agtacggtggcagcgcttggggggcagcgggcaggtacagcgaactggcaggctttgcccattggggtgccaccgccagctggcactctctgtttgaaga
agggcaggccagcggctcgtttgcagaagccggcccttacagctaccctcgcagccatggacctgcgggacccgatggggaatttccctctgacgcctgg
tacccagcaccaaccatgataggcagggtgccctatggtgggcctatgaaggctgaaatgggaccttggatggaaggataccctggagcctttggggaaa
tgaggtgggaaggcaagaatgaaaatgaggtgggacgctgcagtgagactgggaagatccatgtgaaggttgatcacaagtactacaggcctactgaagt
agaattcctaccaggtgactgcagcaaagcaaagaataagctgggctggataccaaaagtctccttccatgaactggttaaagagatggttgaagctgat
atcgaacttatgaagaccaatcccaatgcctaagaagtacatgaatatgacatagcagcctgataaaac
3' EST: CX972256 [+]gacagaaatgaaatttattaagaaaagaaaacctataaaatagctagaaaatcacaaggtccattggcacgactccaccgcttattaataattataataa
ttatatcttcagtatccacctgcgtattttgggcaattaaacaaaaaggcaattttgggaaaaggacaccataaatatatccccattctgacagacagag
gggaaaatcaaatgccagaaaatgccatgttaccagtatgagtaggattaaatattcactgggggagctcagaccttccatacaccttttacttgagcat
ttgtgggggactaagagtccgaagttttatcaggctgctatgtcatattcatgtacttcttaggcattgggattggtcttcataagttcgatatcagctt
caaccatctctttaaccagttcattgaaggagacttttggtatccagcccagcttattctttgctttgctgcagtcaccttgtaggaattctacttcagt
aggcctgtagtacttgtgatcaaccttcacatggatcttcccagtctcactgcagcgtcccacctcattttcattcttgccttcccacctcatttcccca
aaggctccagggtatccttccatccaaggtcccatctcagccttcataggcccaccatagggcaccctgcctatcatggttggtgctgggtaccaggcgt
cagagggaaattccccatcgggtcccgcaggtccatggctgcgagggtagctgtaagggccggcctctgcaaacgagccgctggcctgcccttccctcaa
cagagagtgccagctggcggtggcaccacaatgggcaaagcctgacagttcgctgtacctgcccgctgcccccaagcgctgccaccg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: