XB-CLONE-3554640
Clone Name: IMAGE:7713847
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Gene:
srp54
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972343 [+]
aaaactttgagattgtcattgtggacacaagtgggcgccacaaacaagaagattccttgtttgaagaaatgttgcaagtgtctaatgcagtacaaccgga
taatatagtctatgttatggatgcttctatcggacaagcttgtgaagcccaggctaaagccttcaaagataaggtggatgtagcatcagtcattgtaacc
aagcttgatgggcatgcaaagggtggaggagcgcttagtgcggttgctgctacaaagagtcccatcatttttattggaactggggagcatattgatgact
tcgaaccatttaaaacacaacctttcattagcaagcttcttggtatgggtgacattgaaggattgatagataaagtgaacgaattaaaactggatgacaa
tgaggccctcatagagaaactaaagcatggccagttcactctgagagacatgtatgagcagtttcaaaacattatgaaaatgggtccattcagccagata
ttggggatgattcctggctttggacaagattttatgagcaaaggcaatgagcaggaatcaatggctcgactaaagaagctaatgaccatcatggacagca
tgaacgatcaggaattggataacacagatggtgccaaactttttagcaagcagcccgggagggttcagagagtggcaagaggatctggagtctctactag
agatgtacaagagctgctggcacaatatacaaaatttgctcanatggttaananaatgggaggaatcaaaggcctcttcaaaggtggtgatatgtcaaaa
aatgtaaacccgtcacagatggccaaactgaaccagcaaat
3' EST: CX972342 [+]attttcacactatttattgtactcatgttgaacaaaggtttatgtatggtgtatactgcccctttaacaggactgattatttaaatatatttcggaacaa
gaaatatgaccatttagaaatttatttaacagcaaattgaattccagcaagaggtgtgagtgctggaagcgtatacaaacagtttgtctttgaactaaac
attaacagtttttttttttatacaacagactttgttttgataaaaactaaattaattgtccaataacttaaaatagggtgttgcaccccagttccctgaa
aataaggaatacttggggggagttaaacagtagaagggtggtttctttttatttttaatggaagtagagaatttatagagaccacatttgtctgcccaga
aagcattccagttctttgaagccacagtaataagaaacatccactgtgcaaaaagggcatctttacatgttgttgaatcccatcatccccttcatgttgc
cagcagctccttgttggaactgtctcatcattgactgaagtcctgccatgccacccatgtgctgcaaaactctggggtccatcattttggccatttgctg
gttcagtttggccatctgtgacgggtttacattttttgacatatcaccacctttgaagaggcctttgattcctcccatttttttaaccatttgagcaaat
tttgtatattgtgccagcagctcttgtacatctctagtagagactccagatcctcttgccactctctgaaccctcccgggctgcttgctaaaaagtttgg
caccatctgtgttatccaattcctgatcgttcatgctgtcca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: