XB-CLONE-3554517
Clone Name: IMAGE:7713811
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Gene:
cyp2a6.9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972428 [+]
caaactttgtttattcactccaggcatatggcatctgtggtatcaatatgaggttttaacagtacatcaagcacaattttgcatagcttcacaggcaaca
tataacatcaaactagacttacacttgctatctgtaccttcaaagagatgtcccctgccttagtgcaaatccccagcgtagtcaggcttcacagtaatcc
cttgctcacacactgtgtccctaaacccaaccacttgaataccgcagggttctaaacttttttaagctgctcatgctatgtatctggtgcttgtctttca
ttcctggagtgactatcatagaactaagctaactctacattttctaagcctgtaaccacttctcagccagatgaggtcccaggaaagaccctgagcatac
atgtgctatcccttttacattggagtacatttccacctactgggcatctctaaacttacttttaacctggaaagggaaatagtttttacacacagaacca
ggaccacctttaggtgtccaaaacaaaagaaaactgcctgattggcaaacactgattaatcaggattactacgttccaaacaggtgacaatgggttgcta
tgggtgaccagaccggtagcaaaatgtgtacctattattagattactccctaagacttgagtcccattgtagtttaaaaatagacaggaaaccgaattaa
tgagtaattccagacttcctgacttggcattttaagtaaaatgtgacaaattcataaacatcttaaggataatttatgaccacaaacatggaacactgaa
ttggatgcaaagtagtgcatgaaaaattcagtatgttttgatgaattctgcaca
3' EST: CX972427 [+]tttgatccaccaatatcttggtccatgtgtgtccaatgtaatctgctctgttgtttttgggaatcgttttgaatatgccaacaaggattttcagaatctc
ctttccctgtttcaatctgtcttccaagaaaccagctcttcctggggtcagttgctgaatatgctgccagccgtcatgaatcatgtcccaggtcctcata
aaaatattataagggacatgaataaactggaagattttgttttacaaagggtgaaagagaatgaaaaaactgtggatcccaactcaccgagagaccttat
tgacagctttctcataaaaatgcagcaggaaaacaaaaatcccacttctcccttccatatgaagaatttaatcgctactatcctgagcatattctttgct
ggaacagagacagtaagcaccaccctaagacatggatttctcatacttctcatacatcctgaaatagaagcaaaactacaagaggagattgacagagtgg
ttggtcaaaatcgcagcccgaccattgaggacaggaacaagatgccttacacagatgctgtgatccatgagatccagagacttagtgatgtaatcccaat
gaatgtcccccatttagtcacgaaagacacaaaattcagaggatacacaattcccaagggtactaatatctaccccttgctttgtgctgttcttcgggat
cctgagcaatttgacactccgtccaaatttaatcctaatcactttttagatgacaagggctgttttaagagtaatgatggcttcatgcctttttctacag
ggaagcggatctgccttgnggaaggtttggctcgaatggaactctttctcttcctaaccacattttacagaactttcagttacattcagaatctgggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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