XB-CLONE-3554494
Clone Name: IMAGE:7713909
|
Gene:
tmem33
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX972443 [+]
aattcctatcccgttacaatgagtattttcccagtcttgctcttttccttgcttcatgcatcaacatacacaaagaaggttttagatgccaagggaccaa
acagcgtgccttttctgaggtcttttcttgaaaaactaaatgcaaatcaacaaaatattctaaaatttgttgcttgcaatgagatattcttaatgcctgc
cactgtgttcatgctgcttagtggccaagggagcctactacagcccttcatttactacagatttctaactcttcgttattcctccagaagaaatccatat
tgtcgaacattattttctgagctgcgaataatcctggaacatcttgtcatgaagccagcgtgtccggactttgtgagaaggttgtgtctgagcagcattg
catttataagcaggcttgcaccaacaattgcgtaacttcaatcttgttcagtgatgtatatgtataaacattagcagctggtatttctgctcatagtatg
atacctgggtgcattataccactttcgacctgagcgattccatagaaatcatgttgaactctcttatgcctttttatatgtcacttcttacagtttctta
acccccctatccttgatgtgtttcttttcagtcattactatgtgaggatgctaaatttacaagagacactgtaaagtggttgggatttccccttcctaca
aatgcagggcaaggcgaagcctatttgggcttttttttctttttatactgtatgcaccaacaacataaaaacgcacaacattaacaatcttaacttaacc
tggggacacactctttcagctggatgaaatgattcacc
3' EST: CX972442 [+]gggaggaggtcggacttttattgaaacagaaggctggaattttacaagcagtgtgtaaattattctattctatgtacatctcggtaccaaaggttcagta
caaaggttgaaccttaataaatttatattttaataaaaaataaacttaactacaaccaataccattctgctttcctaccacatttgttgctgaaatggta
aaaaaaaggtgaatcatttcatccagctgaaagagtgtgttcccaggttaagttaagattgttaatgttgtgcgtttttatgttgttggtgcatacagta
taaaaagaaaaaaaagcccaaataggcttcgccttgccctgcatttgtaggaaggggaaatcccaaccactttacagtgtctcttgtaaatttagcatcc
tcacatagtaatgactgaaaagaaacacatcaaggataggggggttaagaaactgtaagaagtgacatataaaaaggcataagagagttcaacatgattt
ctatggaatcgctcaggtcgaaagtggtataatgcacccaggtatcatactatgagcagaaataccagctgctaatgtttatacatatacatcactgaac
aagattgaagttacgcaattgttggtgcaagcctgcttataaatgcaatgctgctcagacacaaccttctcacaaagtccggacacgctggcttcatgac
aagatgttccaggattattcgcagctcagaaaataatgttcgacaatatggatttcttctggaggaataacgaagagttagaaatctgtagtaaatgaag
ggctgtagtaggct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: