XB-CLONE-3554490
Clone Name: IMAGE:7714005
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Gene:
uroc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972447
[+]
agacagtccctacagagaaacttccaatatctatgatgggtccgccttttgcgcagatatggccgtgcagaactttgtgggagactcattccgaggagcg
acctgggttgctctgcataatggaggaggtgttggctgccactgcgtgacggctgttgtcatgaactgggaaagagaattcatcatcatcaaacccgggg
gtccccaaccttttttacttgtgagccacacgcagatatgaaaagtgttggggtgaggtaattaatggaggatttggcctggttctggatggttcccagg
aggctgaggagagagctacaaggatgctgagctgggatgtttccaatggggtggcgaggcgctgctggtcggggaaccaaaatgcctatgatacaattgt
ccgaaccatggaagagaaccggaatctcaaagtgactttgccccatgcagtgaaggacaaaagtgttctggctttggctctgtcccttaattaacactcc
tattccatctgtctacctcttatgctatagaaatcacgttattaagcatcctcttccactcattatataatctagcacgtgtcaatcatcattttatatt
gttgtgcatcagaagggacgctcagtgcatggattacttatgcatttatggtctaactcccccagtgaacccactccagttagtctagatctataatgcc
tgtgcacatactggtacctagaactgtaatgattaaaccttagaacttggacaacctggtgttgcactagtgtttttaagctgtgtgcaccaatattcat
gacattttgctacatttattgtctcattacta
3' EST: CX972446
[+]tttaaacgtttattccccatttaacatgagaaagtattttccccagtagtaatgagacaataaatgtaagcaaatgtcatgaatattggtgcacacagct
taaaaacactagtgcaacaccaggttgtccaagttctaaggtttaatcattacagttctaggtaccagtatgtgcacaggcattatagatctagactaac
tggagtgggttcactgggggagttagaccataaatgcataagtaatccatgcactgagcgtcccttctgatgcacaacaatataaaatgatgattgacac
gtgctagattatataatgagtggaagaggatgcttaataacgtgatttctatagcataagaggtagacagatggaataggagtgttaattaagggacaga
gccaaagccagaacacttttgtccttcactgcatggggcaaagtcactttgagattccggttctcttccatggttcggacaattgtatcataggcatttt
ggttccccgaccagcagcgcctcgccaccccattggaaacatcccagctcagcatccttgtagctctctcctcagcctcctgggaaccatccagaaccag
gccaaatcctccattaattacctcaccccaacacttttcatatctgcgtgtggctcacaagtaaaaaaggttggggacccccgggtttgatgatgatgaa
ttctctttcccagttcatgacaacagccgtcacgcagtggcagccaacacctcctccattatgcagagcaacccaggtcgctcctcggaatgagtctccc
acaaagttctgcacggccatatctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: