XB-CLONE-3554410
Clone Name: IMAGE:7713774
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972501 [+]
gcatgcatatcaaccacaacgtaaaacattacagggccttatgttgttactggggaggttcatcccagacataatgaccaagaaacaatggcagcagaat
tataaataacatggaacattttagggctgtaagagaaacataaagctattcaggcacaacaaatgacagacataacacaaagacaaatctgtgttttaca
aaacttgctacagtgtgcatttacttcacttttacattttcttcttacttgatttgcagtaggctgacagcccaatctctgggtattttttttcttttta
tgattgatatggcatacaggtggtgcaagttgtaatggatactgtggtctgatgacaatttgggtgtatacgttgtttagttttcagtttcagttatacc
tttatttatgaagtcgctaaacttatctcggatgtctttttctaagtattccatttgatcttgcacagtcactgtgtatttcccaaatttcagtttcatg
tcctctaccctctgcactagtgccttgttgagtgcctctccatagggagtaatgctaactttaaattctgcaatcttctgctccatctgctggtttagct
cggagagatatggctctagtgtctgcttagcatccctagcatttacctggagtttctctttcagatcatctgcataggggctcagttgagcttttaactg
ctcagagatctgttttaattgggtgtctgcttgctcaacactctttctcatttggaattccatgctctctatctgcttggctaattctcctgcatctcct
ctgc
3' EST: CX972500 [+]tcacaggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaaggatacggtggacca
gatccagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaagccaagtaatt
ccctttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtgaagatttctc
cttacgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagccaactagagaa
gaacactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatctttggcacca
tattcagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataagattggccatc
aagtacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattccaaatgagaaagag
tgttgagcaagcagacacccaattaaaacagatcactgagcagttaagagctcaactgagcccctatgcagatgatctgatagagaaactccaggtaaat
gctagggatgctaagcagacactagagccatatctcaccgagctaaaacagcagatggagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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