XB-CLONE-3554314
Clone Name: IMAGE:7713926
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972566 [+]
acaaccctcgctctggttttatgtgccatcacaggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgc
aaagcagcaccaaggatacggtggaccagatccagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaaccttta
tgctggagaactacaaagccaagtaattccctttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggag
ctggagcagctcagggtgaagatttctccttacgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatg
ctgaggaactgaaaagccaactagagaagaacactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgc
aggaaatttgcaagaatctttggcaccatattcagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacact
gatgaagtgagagataagattggccatcaagtacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatag
agagcatggaattccaaatgagaaagagtgttgagcaagcagacacccaattaaaacagatcactgagcagttaaaagctcaactgagcccctatgcaga
tgatctgaaagagaaactccaggtaaatgctagggatgctaagcagacactagagccatatctcaccgagctaaaccagcagatggagc
3' EST: CX972565 [+]ccacaacgtaaaacattacagggccttatgttgttactggggaggttcatcccagacataatgaccaagaaacaatggcagcagaattataaataacatg
gaacattttagggctgtaagagaaacataaagctattcaggcacaacaaatgacagacataacacaaagacaaatctgtgttttacaaaacttgctacag
tgtgcatttacttcacttttacattttcttcttacttgatttgcagtaggctgacagcccaatctctgggtattttttttctttttatgattgatatggc
atacaggtggtgcaagttgtaatggatactgtggtctgatgacaatttgggtgtatacgttgtttagttttcagtttcagttatacctttatttatgaag
tcgctaaacttatctcggatgtctttttctaagtattccatttgatcttgcacagtcactgtgtatttcccaaatttcagtttcatgtcctctaccctct
gcactagtgccttgttgagtgcctctccatagggagtaatgctaactttaaattctgcaatcttctgctccatctgctggtttagctcggtgagatatgg
ctctagtgtctgcttagcatccctagcatttacctggagtttctctttcagatcatctgcataggggctcagttgagcttttaactgctcagtgatctgt
tttaattgggtgtctgcttgctcaacactctttctcatttggaattccatgctctctatctgcttggctaaattctcctgcatctcctctgcataagggc
tcangctctcacgtaacttttgtacttgatggccaaatctatctctcacttcatcagtgtaagggttaagttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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