XB-CLONE-3554311
Clone Name: IMAGE:7713974
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Gene:
adat1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972568 [+]
cattttcgctgctctaaacatgcaagcgaaggggcaggttatgaagagagaaatgtttcctgtttgtgtgtctgtaacttttggttgccacttggctggt
atgaatgatacttgtcttgttattaatagggatgtaagagaatatgttaaggaaatctagagaaggtggcaaccaatagtgtaactttaaagggcaagtt
agcataacaactccctgtattctgttatgtatgtgacataactcatgtaacaaactctgctattgatatggttaatgttttgataaatgttataacatta
atcagtacatcagtaatgtaattcacagcactgctgtacaggttgcagggaaaaccagagcctatagtgcctgtgaggcactatggggtctatttttgaa
actagcactgacttcatgtacctgaaaaaagttatttgattatattattgctataaacctttatgtaagtacccatgtaactgttaatgtgcctgtcatt
tttaaaattatccacgtcagtgtttcttttctcatctgatctttgaaggttatggtctgcagacgagattgctgctttgagttatggacattacactact
cagttacctaagcagggattacctgatccaagcagagaatggacgctgatggctgcagtaatacaaatagagtctgtggaagacacaaaagttataaaaa
aagttgttgctatgggaactggaactaaatgtattgngcaagcaaagctacgaaagacaggtgatgttctccnagacagtcatgcagagataattgcaaa
aagaagttttcaaagatatctcctt
3' EST: CX972567 [+]tccttccctgccaatcactcaaagcatgtccagtctgcagttagctgaacataaggaaatcacgaggtgtctggatccaagatgtgaaagcttgctgtct
caaacaattccaggcttcctggtaagtgatagctgccgccttataatcccagtaggtcttcagtccttggctaagagactctgagcgctgtttctctgac
agcctctgcaccagctcccgaaatgtgttgaatatttctgctttacagattcgagacctgcactgagggcttccaattgcttttctggttgttccttgcc
tgtagccatttgcagtgacatctagtggatggtggggtactgcactccaactaacagctgctccacatggaacaagtttcctggtagcatcctttttagt
cagtgcatgccttccatgttgaaattccaagtctgactggatgatttgaaccctattcaatctaaatgcacaaggcagagataacaccttatggcatctg
caggaaataggacatgatagtgattacatcacatcatatatacattacattattttttatggcattaagggaggcatgacatcctcttatttttacctgt
tatagagagctctctccatagcgtcttggctgaatggacactttccaaccacaactgcagatagatagattggctgctgcagaaagtgcatgagcagagc
accctgacagccaagtacgttccagcgagccattttgtcactacaggacatggacatggtacggtctccgcgacctggcttgatacgga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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