XB-CLONE-3554279
Clone Name: IMAGE:7713786
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Gene:
pnpla3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972590 [+]
tttttttattaaccttttattaagtctcggtaagtgacaaagcgcggtggattctctggcagcaaagggttaatctttattgaaaaagactaaagtacta
aaaaaagggtcccattcacccaaagtgctcgatcgggtcataaagctttggaattctcaataaaactcactgtacatataaaactctcttttaaaatttc
acaaaattgtatttctttgttatagatcatttaaagctggaagcaccaaacacgatgtgcctttaggagaagaaggggttaaacctcaggaggtgaaatg
gggcaaataagaacccgtggtgcttaaccgtctaagtgccagagggctgagagcattgcaaccaacgcttagtaagtagatcaaacggacgcagagatta
aataaatgctggttgggggtcccccaaagacatgtttgggggtacccgatatacctgatattggaagggttatgtcagaccaataacgtcgcgaaaaaat
ccccccaaatctgctatatatgatagatagatagatataaagataaatagatagatagataggctataaagatagatagatagagagagagatagataga
taggctataaagatagatatagagatgattgacagatatagatagatacatatatagatagacagagatagatatagagatgattgacatagagatagat
agatatagagatagatagaatttttgggggacattattggtttgacacttccaaaagtccaatttatcattgttatttactccagaacttggagtcgtct
tatccagggtcagactggccccccccaagacaaatcccac
3' EST: CX972589 [+]ggctgggtcttcgacttctcctgtccctcactcctccgggaccaccaggaccccaacgatccccaattagaactggattccgacgcccaacccaagtgca
ttttcagcttggaagactcaggggtagatgtgaacttctccaccgatccggaagattcggcttcggaactgtaatttctgggttttactggaaattctca
ttactcccttcagatctggatcctcaggttataaaggaaggtccctaatgatccggagaaccccgattgattcagaattaagtttttggggttttaatgg
gggagcaaattggacctcggttttcattttgtcgtgaaactaaaaaagttgatgggaccagcgtcggactgggttactggggcccagcaccaccaacccg
gcgctcacccgagacagcagttggcctggttctgttgggcccagtgggatttgtcttggggggggccagtctgaccctggataagacgactccaagttct
ggagtaaataacaatgataaattggacttttggaagtgtcaaaccaataatgtcccccaaaaattctatctatctctatatctatctatctctatgtcaa
tcatctctatatctatctctgtctatctatatatgtatctatctatatctgtcaatcatctctatatctatctttatagcctatctatctatctctctct
ctatctatctatctttatagcctatctatctatctatttatctttatatctatctatctatcatatatagcagatttggggggattttttcgcgacgtta
ttggtctgacataacccttccaatatcaggtatatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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