XB-CLONE-3554224
Clone Name: IMAGE:7714030
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Gene:
ctrc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972628 [+]
ccgaggcccaaagttaccagggttgtcggtggagaagatgtagtgccacacagttggccatggcagatctccctccagtatcagggaaccagtggtgctt
ggggtcacacctgcggcggaaccctcatctctgaacagtgggtcctgactgctgcacattgtatcagctctggccgtgtctacagagtgttcctgggcaa
gcacagcctgcagcaggatgaggctgaagccgtcgctatctcccctgagaaaatcattgtccatgagaagtggaattcccttttcattatcaacgacatt
gctctgatcaagctgtcccagcctgctcctctgagcgaagcgatccaaccagcctgcattccttccagcggtgccatcctcgccaacgacttcccctgct
ttgtgactggatgggggcgcctctacaccaacggaccaatcgctgacaacttgcaacaagcccttctgcccgttgtggaccatgccacttgcaccctgcg
tgactggtggggaagccaggttcagaccaccatggtctgtgctggtggtgatggaattgtgtctggctgcaatggtgattctggtggccctctgaactgc
caggcagcaggtggagcttgggaagtccacggcattgtcagcttcggctctggcattagctgcaactatgccaagaaaccaaccgtcttcacccgtgtgt
ccgcctataacgactggatcagcgagaaaattgccagcaattaagaacaagcgcaatttccatcgtatccgcaacaacagggcatctgattcgtgcattc
ctcgtatttcctttaacacctcgtgtcatacacattttgga
3' EST: CX972627 [+]ctctttgctttgtcactttattgcgaaatccaaaatgtgtatgacacgaggtgttaaaggaaatacgaggaatgcacgaatcagatgccctgttgttgcg
gatacgatggaaattgcgcttgttcttaattgctggcaattttctcgctgatccagtcgttataggcggacacacgggtgaagacggttggtttcttggc
atagttgcagctaatgccagagccgaagctgacaatgccgtggacttcccaagctccacctgctgcctggcagttcagagggccaccagaatcaccattg
cagccagacacaattccatcaccaccagcacagaccatggtggtctgaacctggcttccccaccagtcacgcagggtgcaagtggcatggtccacaacgg
gcagaagggcttgttgcaagttgtcagcgattggtccgttggtgtagaggcgcccccatccagtcacaaagcaggggaagtcgttggcgaggatggcacc
gctggaaggaatgcaggctggttggatcgcttcgctcagaggagcaggctgggacagcttgatcagagcaatgtcgttgataatgaaaagggaattccac
ttctcatggacaatgattttctcaggggagatagcgacggcttcagcctcatcctgctgcaggctgtgcttgcccaggaacactctgtagacacggccag
agctgatacaatgtgcagcagtcaggacccactgttcagagatgagggttccgccgcaggtgtgaccccaagcaccactggttccctgatactggaggga
gatctgcccatggccactg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: