XB-CLONE-3554122
Clone Name: IMAGE:7713944
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Gene:
etnppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972696 [+]
ctttaagcccatttgcattgctttaaaatattatttagcagatatacaatgtaaataagattgtacaaaatatgtggatataatttattatagacaattg
tgtttagtcctttattaacaaaaaacattttctataaataatttaatttttttccagctagcgaaagtgaaagtcttttgtactgaccacaggaggataa
acttagatctgatatcttcttttatatagattgctgcttttattgctgagtccatgcagagttgtggaggtcaaataatcccaccagcaggctacttcca
gaaagtgtcagagtatgtacagagcctaatgagaaataatggcttttctcggttcacttaatctagcttttctatcttatagcacattaaactcatacag
tccaaacaattacaaaacatataggagctgaattactaacagaagttctaaactgcactagagcagttgcaaatagcagccaatcagcaatttgttttaa
tcagtctactacaagttagaatatgaaagcaaaggtctggtgatatttagcttctttgttagtaagtcagccccataattgtgacactattgtgagatta
tactattcctacagaatgcaatacaaacttggtgtagacatttattgtatacaaacacaatgttaagaagaagaaactttccaatcattcattgtctttt
ttatgtttaccttcattctcttgactagtttctatacatctttctaccaaccttttcagaattcctatgtttcctctctctctctctctctctgttttta
tatcatcatcattgtctggaaataggtctgcaaagctcattcactaatatgtgcaagataccatataagtctagttt
3' EST: CX972695 [+]gcagaatttaatgaataagcttttatttcttattatgttttgaatctagtttatagagaacaaatgatatactgtagatatgcatggcatggatttagcc
aagcagtaggatctggtataatgtgattatggatatctgctcttctggcactctttcatagtcattcttgtcaagtgctgttatacatctgaaatgttct
gacactttaagaaaagaagcatacgcatgtttacctagttttaagaaaactgctgatcccccatgtagcttctcattttaaaatataaaacattatagca
ccttgtatgttgacaaaaaagaaaataccataggcagtgagaaaatgcccccttatgctattctatggggctcatattcaagtaaaagggatataggaat
catgtacacagggatttcaggctgctacatcagatctgtagggacccctaataaaaagtaaaataatgtagcgagtggaaaagggttacttatttgagca
agagctaaaatcttggtgtatctaaacagttggaaacggcttaaaattttcttgtacatagcatgctcagtacattcagcatgctgaggtcttttaaact
gcatcttaaatctaagccattaaaacatgggaaaagattccattccaatatgatatactgaaactgcaaatacagaataaaaaaaaataaaaaggatgca
ccacataaacccctaaaattcattggcaaatcaaatttaacaacccttcgccagactagaccaagcatcttcatttgttagagttgttagaataggaagg
cagcaacagtttgaagattctgaactcagaaacctccttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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