XB-CLONE-3554098
Clone Name: IMAGE:7713946
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Gene:
syap1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972712 [+]
atttgtctgtataaacagatcataaaccaaactggaatatacatttaaacttgtgcagaggaaaatcttaattgattaaaatcaggtataatgttcttta
tggcagggtaaaacaaaaatatgggccaaaaaaaaaaaaccccccaagaaacaaacaaatccagacaggataccatatacctttcaaatcacatagaacc
aggcctcactcattttgtacgtattggaatggaaaacaaatgcttcgttttgaatggtgaaaaagaaatcgtgttccaataatttggcaccacacacctc
tgtggcaaatacttggtcttttagcttttacttgtactagagagacatatatatgatgaaaccaccggcgcctctctggcaaagactttcttcccaggct
ggaaataaatacgttaaattacatgtcagctctacttcagtccatatcccagataaatgaataagaccgctgtgttgttacattgtgaagtgctttttaa
aatagattattacagtcacaatccagaagagacagggcttcacagggcttcccagaacttcagtttaatagcacttaatcttccattgctagttctaaac
agtagcgccttagcagtacatagcgaaaacagccatgatcttattctgtggtttgcagagcctgaaggaatgactagagtaggaagagaatagtatagac
acagtccaactgatcagcattacgttagtcctcttgtaggagttcctcaatctctttgtcccaattttcatccttcattttgtcagagtctgtcaccacc
tcatattcttgaaccacatcctgtaactcctttatccagtcctcggtttcttcttctggtttatc
3' EST: CX972711 [+]ctggacccggagcaggaaacacaaaaaacagcagagaaggaaacaggaaggaatgaagaagagactagaagcgcggaaccaggagacggggaaaagttga
aaagcgctggggaagatgggcagtctcagcaagaggcaaatgagtcagacgaggccctcataaaccaggccaaaggctttggcagttaccttttaaactt
tgcttctgttgctacaaaaaagatatctgaatctgtagccgaaacggctcaaaatatcaagaaatcggttgaggaaggaaaaatcgacggtttaattgat
aagacgatcattggtgattttcaaaaggagcaggaaaaatttgtacaagagatgcattttaaaaagtcagaagctgcggttgctccttgggttggttata
atgaagaggaaaccattcaacaacaaatactggccctatctgcagacagacgcaatttcttaagagatccccccgctggagtacattttaactttgagtt
tgagcaaatgtaccctgtcgcccttgtaatgttacaggaagatgagctacttaaccgaatgagatttgatcttgtcccaaaactcgtaaaggaagatgtg
ttctggaggaactacttttaccgtgtttctttgattaagcagtctgcacagttaacagccctggcagcacagcagcaagcaacaggaaaagctgataaga
ttaatagcatgcaagatggcggtgacttagaagaaactgtaaggccaaagactccaccagtgactatcaagtccccacttaaaaatccagaggaagaaga
ggagatttctacaagcccaggagtgtca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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