XB-CLONE-3554089
Clone Name: IMAGE:7713708
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Gene:
hnrnpa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972718 [+]
ctgtttattggaggcctgagttttgaaacaactgacgaaagtctccgtgagcactttgagcaatggggtaccctaacagactgtgtggtaatgagagatc
caaactcaaagcgttcccgtggctttggatttgtaacctactcttcaacagatgaagtagatgctgccatgtctgctcgcccacataaggtggatggacg
agtggttgaacctaaaagggctgtctctagagaagattcctctaggcccggtgcacacctcactgttaagaaaatctttgtaggtggaataaaggaggac
acggaagaacatcatttaagagaatattttgagcagtatggaaaaattgaagttattgagataatgacagacagaggcagtggcaagaagagaggctttg
catttgtcacatttgaagatcatgattcagttgacaagattgtcatccagaaatatcacactgtcaacaaccacaattgtgaagtgaggaaggccctctc
aaaacaggaaatggctagtgtttctggcagtcagcgaggacgtggtggctctgggaacttcagtggtcgcggtgggtttggcaatgacaacttcggtggt
cgcggtggaaactttagtagcaacagaggtggtggatttggtaacagaggttatggtggagatggctacaatggctttggcaatgatggtggttacggtg
gcagccctccatactctgggggtaacagaggctatggtggtggtggtggccaaggctatggaggtggccaaggaggtggatatggcggcggaaatggagg
ttatgatggctataatggtggaggcagtggctt
3' EST: CX972717 [+]gaaaaacagagctacacagtagctgtggaattggaagttggtcagatctgtcacatcaggtaatcttgaaacctgcatcaggacacttcttcccaactaa
ggtcccaggtcagtttaatcatgtcgaaggatattctcctccatatggtccagaattcctgccgccaccataattgccacctttcattgggccaaagctg
gatgatgactgattgttgtagttgccaaagtcattgtatccaccgccaccaccaccactgccaccaaagttaccaccagagccaccgaagccactgcctc
caccattatagccatcataacctccatttccgccgccatatccacctccttggccacctccatagccttggccaccaccaccaccatagcctctgttacc
cccagagtatggagggctgccaccgtaaccaccatcattgccaaagccattgtagccatctccaccataacctctgttaccaaatccaccacctctgttg
ctactaaagtttccaccgcgaccaccgaagttgtcattgccaaacccaccgcgaccactgaagttcccagagccaccacgtcctcgctgactgccagaaa
cactagccatttcctgttttgagagggccttcctcacttcacaattgtggttgttgacagtgtgatatttctggatgacaatcttgtcaactgaatcatg
atcttcaaatgtgacaaatgcaaagcctctcttcttgccactgcctctgtctgtcattatctcaataacttcaatttttccatactgctcaaaatattct
cttaaatgatgttcttccgtgtcctccttta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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