XB-CLONE-3554060
Clone Name: IMAGE:7713854
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Gene:
sgk2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972737 [+]
ctcctttctcagactaggggcatttaaaccctcacaatcgagaggcagccccaaagcacctcttcttttgtgccattgctctgggtcttatctgctgttt
gccgcactgcgatgtggcggggcatggtgagaggacttaaactgatgactacttgatcttagtcttatggaagcataaggaaaggctccaaatactccga
ccccattcattggcagctggcatttggtacctaataaggaatgagctactataatattatattgtgttttccattactgcctttaccccttcatcccact
gccatccccttaccttgatttattttacctaataacaatattttatgtagggttacttatgtatcagtctggaggggttggacataccaggagaccttag
acacaaaggtcactaaacaagataccttcttcattattatctctttcttctttcctccagttctgtttttcacctcttcatctgctttcaccctcgggca
caaactacctcttgagccagtactggttttccttctttgtgatagctttctagttcctgttcctaacccttcctcatactgtcccttcttccaccctctc
tttctttttgtcccctcgctcttcttttctcagagtttctacctttctttgttttgtacctaactagtttggggaaccaggtaataatataaatgtctat
cctcctgctgtctggttgactaatcctggcaaccaggctaaccagagaatagaaaggcaaatcatttaaaaaaacaaagaacaagtaactgtggcttaga
tgaatccatgtagaacagtacattgcaagctcacattgaccacatttacacataaatctggcaggcagcattt
3' EST: CX972736 [+]tataaaaatatagtccttggtttcaggcacaatatgcgcagaccttgcctccaaaaatgaaacggaatgttgaaccaggaggaaaatatttatttcgtga
aaatcgacgtttttgtgttgcagctgagcgggtcgtgatgggataaatgaagtgttttggtagaaaaaggacagaactgagtaagtgatacagtgggtta
tgggtaacgtcgctgagtttgcctaaggatccagttaggcagatatcatagagctgaatgccccctgcataccatggttgctattgtaaggggcaactcc
ggcttcagaacctaaattagtggggaggaacagggggcggggcttagggaactgttccaaaccatattattacattaaaaatcattaataggctgcattt
ttttaaactgatgtatattgcaaagttgtttaaaattatgtttacttttcttaaagcttaagttgtttttaggtggagttaagtaggctatcagtaactg
gctgctgtgaactgaatcacaagcagccaatagcttatcttattatgtgcctcttgggcctataatctgtaaagagcagtgtcggactggcccatcagga
tacccagaaaactcctagtgggcccaggtgtcagtgggcccttctgcctgatcatttgtcctgcttcgtggtcattccctatttctgaatcggaacaaag
tagagaaatagatggagaagtaaattctagcatgtaanagagactagggggcagatttatcaatacacaagttcgaatcccgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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