XB-CLONE-3553987
Clone Name: IMAGE:7714004
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Gene:
uroc1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154999
[+]
AGACAGTCCCTACAGAGAAACTTCCAATATCTATGATGGGTCCGCCTTTTGCGCAGATATGGCCGTGCAGAACTTTGTGGGAGACTCATTCCGAGGAGCG
ACCTGGGTTGCTCTGCATAATGGAGGAGGTGTTGGCTGCCACTGCGTGACGGCTGTTGTCATGAACTGGGAAAGAGAATTCATCATCATCAAACCCGGGG
GTCCCCAACCTTTTTTACTTGTGAGCCACACGCAGATATGAAAAGTGTTGGGGTGAGGTAATTAATGGAGGATTTGGCCTGGTTCTGGATGGTTCCCAGG
AGGCTGAGGAGAGAGCTACAAGGATGCTGAGCTGGGATGTTTCCAATGGGGTGGCGAGGCGCTGCTGGTCGGGGAACCAAAATGCCTATGATACAATTGT
CCGAACCATGGAAGAGAACCGGAATCTCAAAGTGACTTTGCCCCATGCAGTGAAGGACAAAAGTGTTCTGGCTTTGGCTCTGTCCCTTAATTAACACTCC
TATTCCATCTGTCTACCTCTTATGCTATAGAAATCACGTTATTAAGCATCCTCTTCCACTCATTATATAATCTAGCACGTGTCAATCATCATTTTATATT
GTTGTGCATCAGAAGGGACGCTCAGTGCATGGATTACTTATGCATTTATGGTCTAACTCCCCCAGTGAACCCACTCCAGTTAGTCTAGATCTATAATGCC
TGTGCACATACTGGTACCTAGAACTGTAATGATTAAACCTTAGAACTTGGACAACCTGGTGTTGCACTAGTGTTTTTAAGCTGTGTGCACCAATATTCAT
GACATTTGCTTACATTTATTGTCTCATTACTACTGGGGAAAATACTTTCTCATGTTAAATGGGGAATAAACGTGTTAAA
5' EST: CX972787
[+]gtaacaagtacagtaacggaaactcctattgcagattctaaacatggagcaggagcagaagactgtggtgaatggttcagccgacgtcactgttgccata
tcggaccccaattcaaacagtaaagaaaagaaaggatttttctcaagattcaaaaaaaaagaaaaaactgagaaacccccaacggttggagtgtttacta
tgtttcgctattctagtacatcggatcggattttaatgctgttcggtaccatagcatcgatttcccatggggctgctttgccgctaatgatgattgtgtt
cggggacatgacagacagctttgttaatgtcggacaggttggttcagggaacttttcgttggaagctttgataaatgcctcaagaaacctgcaggaacaa
atgaccacatatgcctactactattcaggcttgggattcggagtgcttctttttgcctacgttcagatttccttctggacactctcagcaggcaaacaga
ttaaaagaattcggagcaactttttccatgcagtgctgcggcaggagattggatggtttgatgtgaacgacgcaggagaactgaacacgagactcacaga
tgatgtttccaaaattaacgaagggattggtgataaaattgcaatgctgctgcaatcattaactacatttgtgactggctttattattggctttgttaaa
ggatggaagctaactttggttatcttggctattagtccggttatgggattttccgctgctatctgggcaa
3' EST: CX972786
[+]gtttaagcaaactttaatcaaggacttgcttgattatagtaaaaacaaacaaatacctggaacgtaagttggagtgcttgacccgcatcacataaaaata
attatcaaagaactgaggtaacgtatatagaaaaataagaattgtatacaaaagtattgataaggcaaagaattagtatacattccctgaaataacaaaa
acaaaggtttcttttttcccatccgtagtagtcacaagtgacagagcagtagattccttactgtttagcagcaatggggcaggaaacagtatccaccttt
cctagtggcgtttcctaggcggttttgggttggaaatggcacttctctctcacgagtggcccagctgtatagtcactaaagtgtagtaaacgcccttgag
ctgaaggagctgctgatgtgtcccttgctctacaacctttccattttgtatgactgctattttatctgcattctgaatggtggatagtcggtgggcgatg
acaatacatgtgcggcccgttctggctttgtccagtgcttcttgtacaaccttttcactttctgtgtccagcgcagaggtagcctcgtccaacagaagga
ttttgggtttccgaaccagagctcttgctatagcgatacgctgcttctgtccccctgacatttgggtgcctttatctcccacacgggtgttgtatttatc
agtaagagtgtcgatgaagctgtggatgtttgcttccttagctgctgtctctatctctgcttgggtcacttctctgctgttatctccgtaggca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: