XB-CLONE-3553960
Clone Name: IMAGE:7714054
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Gene:
dse
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC160517 [+]
ccgaacccgagcatgaccttctgtctcctgggggcggtaccgagaggccagggatgatctgccctgactcccagacgcgagagatcgcaagcacatccca
gaaagtgcagtgtgggtcgctctgatttctctggacttggctcagagagtccggctgtatcaaagatggtctggcagagctggtggcctggagatctaat
gtcacaatgaggactcacactcggggagcccccagtgtatttttcatacatttgctttgttacattgtggtttactgcacagaaaaggacataaatacac
aaataccattccttaatgcaaaatatgatggctatcccatgctatacttctccaagggtgagatggaaacacttcgagcccaggcagcgagcagccatca
gcacatttctgcaagaattaatgaggcagttcatgtaatgctgaccaaccctagtgagtaccttcccccatgggatccaaaggagttttctgctcgatgg
aatgaaatttacggcaacaaccttggtgcgctggcaatgtactgcgttttgaatccagataacactgaagcacttggcctggccagagaatatatggaaa
ggatggcagctcagcctagttggttagtgaaagatgctccatgggatgaagttcccttggcacactctctggttggctttgctacagcttatgactttct
ttacaactcctttagcaagcaacagaaggtgaggtttctagaggtaatcgccaacgcctccgtaaatatgtatgaaacctcattcaggagaggatggggc
tttcagtacattcacaaccatcagcccaccaattgtgtggccctgctaactggaagtttggtcatgatgaatcaaggatacctgcaagaggcctattttt
ggacaaagcaagttctgacaatcatggagaagtctatagttctattaaatgatgtgacagatggctctctctatgagggagtggcttacggcagctacac
cacccgatccctttttcagtacatgtttcttgttcagagacacttcgacattaatcacttcggccatccttggctaaaagagcactttgcattcatgtac
agaaccgtacttccaggctttcagagaactgtggctatagcagattccaattataattggttctatggacctgagagccagctggtgtttttagacaaat
atgtcttgagaaatggaagtggtaattggctggctgaacagattcatgcaaaccgagtgcaagaaggtcctggaacaccagctaaaggacagcgctggtg
cacccttcatactgagtttctttggtatgatgcaactttacagtctactcctccacctgattttggcacacctcagctgcactactttgaagactggggt
gtagtgacttatggaagttccctacctgcggaaattaacagaccttttctttcctttaaatcaggaaagcttggaggccgtgctatatttgacattgttc
acaaaaataagtacaaagactggattaaaggatggagaaattttaatgctggacacgagcatccagatcaaaactcttttacatttgctcctaatggatt
tccctttataactgaagctttatatggaccaaaatatactttcttaaataatgctttaatgttctcgccttctgtgtccgagagctgctttgctccatgg
caaggtcaggtaacagaagactgtacttctaaatggctaaaatacaaacaaggggaagctgcagactctcaaggaagggtggtagctgctgtggaaaaat
ctggggttgtttttatcaggggtgaaggggttggagcctacagtcctaacttgaaattgaaaagtgttcaaagaaatcttatcctgctacatccacagtt
gttacttcttgtagaccatattcacttagaccgtggcagcactctggaagcaacttccagctttttccacaatgtggatatgccttttgaggcaacctcc
attgatagtgtccatggagcaataattaggcacagagatggaatgtataagatgtactggatggatgacactggtcagagtgagaaagcagtcattgctt
caacttcttacccacagggttatccatacaatggaactaattatgtgaatgtcacaacatacctcaggaaacctatcacaagagccatatatgtttttat
aggaccttctgtggacgttgagagctttagtgtccatggagattatcagcaagttgatgtgtatcttgctaccagtgaacatgcatatgctgcttacatt
ttcacaggagaaacacctagccagtccatctatgctaaagtcgttgcagatagacagaaatttgtctttgacaagacctcctccatcgggagctcttctc
cagaggaagttaaagattattctgcgattgtagagcagaatctccagcactttaagccggtttttcaggaaatggagaagcaaattctgtctcatgttaa
aaacacggccagctttagaaagacagctgaacgtctgcttaggttttcagataaaagaaacacggaggaggctattgaaaaaatattttcagtatctcag
caacagaagcagcaaggcaaaaccaaaaaaatcaggaaggccacaagaaatgacaaatttataaatgctgtaccagatttatttgcacaaatagaagtca
atgagaagcagacgcgccaaaaagctatggctctggcacagagtgaacttccagtcaatgaagaagaagaaatgaaagaccttttggactttgtagataa
acctcaagtcaagcaaaaatctgtttctgttagcaaacatgtatcccctcacatgttaacgactttccacagcgggacttctatttcggactcctacacc
agactgtttttaattctaaacatttccatcttcatggtcctgttagtgctacagctatctcggttcctgaagccaaagggtatgcaacagaaaagatgtc
tgtatgctatactcacattggatttttgtatattgatgtggctgtatttatcatgttatcagtctcaatgttagatggcagacatgtcaaattgcttcaa
ctgttaagcagtaagtgtctggtgtatacttgaaatgaatgcttaacatagctgtagggctacaagtttgccttcccttacatccgtgctgaaacacaca
atggccagtgtgtggagagagtccgtaatattcaaattcatctacagcacagaaattcaaaaatcactttttagggggtttatatttttataaagaaaag
gggtatgagaaggaaaagtgaataaaattgtaactgtacctactcctctgtcatgaataaaatcagtttatcatattaaagtataatttaaatcaaaaaa
aaaaaaaaaaaa
5' EST: CX972805 [+]ccgaacccgagcatgaccttctgtctcctgggggcggtaccgagaggccagggatgatctgccctgactcccagacgcgagagatcgcaagcacatccca
gaaagtgcagtgtgggtcgctctgatttctctggacttggctcagagagtccggctgtatcaaagatggtctggcagagctggtggcctggagatctaat
gtcacaatgaggactcacactcggggagcccccagtgtatttttcatacatttgctttgttacattgtggtttactgcacagaaaaggacataaatacac
aaataccattccttaatgcaaaatatgatggctatcccatgctatacttctccaagggtgagatggaaacacttcgagcccaggcagcgagcagccatca
gcacatttctgcaagaattaatgaggcagttcatgtaatgctgaccaaccctagtgagtaccttcccccatgggatccaaaggagttttctgctcgatgg
aatgaaatttacggcaacaaccttggtgcgctggcaatgtactgcgttttgaatccagataacactgaagcacttggcctggccagagaatatatggaaa
ggatggcagctcagcctagttggttagtgaaagatgctccatgggatgaagttcccttggcacactctctggttggctttgctacagcttatgactttct
ttacaactcctttagcaagcaacagaaggtgaggtttctagaggtaatcgccaacgcctccgtaaatatgtatgaaacctcattcaggagaggatggggc
tttcagtacattcacaaccatcag
3' EST: CX972804 [+]gatttaaattatactttaatatgataaactgattttattcatgacagaggagtaggtacagttacaattttattcacttttccttctcataccccttttc
tttataaaaatataaaccccctaaaaagtgatttttgaatttctgtgctgtagatgaatttgaatattacggactctctccacacactggccattgtgtg
tttcagcacggatgtaagggaaggcaaacttgtagccctacagctatgttaagcattcatttcaagtatacaccagacacttactgcttaacagttgaag
caatttgacatgtctgccatctaacattgagactgataacatgataaatacagccacatcaatatacaaaaatccaatgtgagtatagcatacagacatc
ttttctgttgcataccctttggcttcaggaaccgagatagctgtagcactaacaggaccatgaagatggaaatgtttagaattaaaaacagtctggtgta
ggagtccgaaatagaagtcccgctgtggaaagtcgttaacatgtgaggggatacatgtttgctaacagaaacagatttttgcttgacttgaggtttatct
acaaagtccaaaaggtctttcatttcttcttcttcattgactggaagttcactctgtgccagagccatagctttttggcgcgtctgcttctcattgactt
ctatttgtgcaaataaatctggtacagcatttataaatttgtcatttcttgtggccttcctgaattttttggttttgcttgctgcttctgttgctgagat
actg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: