XB-CLONE-3553847
Clone Name: IMAGE:7714257
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Gene:
trappc8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972884 [+]
ttattgaaaagcatattatattgaggtgatcaactcaatcgttagagttaaataataacaaagtcgtgaaatagtgcactgagtattgagatgaatctcg
ttgttaacaaatgggcttaagaatatcacttagaaatgggagtttgctgattggctaatcaaacggagcagcgagcgaaaaatttcagtactctgaaaca
aaccagtaagggaaaagaagaactgacaaaaccaaagggggcaaataaaaggaacacagcagaacgtatagaacataatgagcggttgtatttctagttc
tctttctgtatttacatctgtaaggaatgcatccggcgctaaacaggaaatacctatttccttggcaaggctgaaagtaatgctctgtacagatgaatga
aatgggcagctgatttgtcagttccagatatcttgcatatagaaactgtaactattcaactgaacatttacttttggttcctatggaatctcctgcatgt
cggatattaaaggaggtgtgactttcatccctttattccaagaaaacccagtatatctgacattaaactgaccgattcattcccattgtgcttggggtgc
aaagccgtcagacactgttaataacaataagagctggcattgaactctgctgattggcttcaaacagcgtgagttgattggccagcttagcanacacccg
aggagtcccaaggttgtaaattcccgtgtgcaaaaagcaagccttgagctgcaatgtgaagacttcgtggctcctcagctgcagtttatattccgtttgc
cc
3' EST: CX972883 [+]gagaggcagagagtgaaggtggcaatatggtagtattcatggatgtggaaaatatgaatactagtgaagcgggtgtgaaagaatttcatgtagtgcaagt
atcaagtagcagtaagacctggaagctccagaagtcagtcaaccctacagatgacaaggatactaaattaaccagcagagagagggccaaaatgtgtttc
aaagctgtaagatgcaaatcatctttgggttctgccccacagacggaaagatatacatttggagacattgtttttggaggtgaacagatcattagttcaa
ctactccatgtgcagatttttttttccaaagctgcttaaggcaacagcttgataaaaatccgactgcaaacacagatgggtatggaaagtcaacggccga
caagactgtcagactgatacagaagtgcagtgaagttgacctgaacttgatcattctgtggaaggcctatgtcattgaagataataagcagcttatcttg
gaaggccagcaccacgtgtccctgagtactgttggggcagaagctttgtcttttcctcagaagcaggaacagcccgaaatggttctcttaaaattcactc
ggtctgaaaataatgctgcacttgtcacaccatcaccagaacagctttccaatctaataaaaacaagtcttcactacccagaatctttcaatcatccatt
ccaacagaaaagcctttgtttagttccagtgacactggtgctttccacagttcgcaggctgaagtggatgttattgttgacctaaggcataaggccagaa
gccctgaagctct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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