XB-CLONE-3553770
Clone Name: IMAGE:7714217
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Gene:
clk1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972935 [+]
tgcactacagcaaacgaagtagacgtccagacgaggatgagaaggagtggtatttcagcaagtgccacaaacgaaaaagatcacaaagcagcgatgaaga
aaaggaacgccataaaaagatctcatcatctgacagccactacgtggaggacaagtccatgtgttctagaggtcagcatgacagacgatatgtagaacta
agaaataactacaagggtggacatcatgaacatagacaccactacagatccagcaagtcatctgggcaaagcagcagcagcagcagcagtcacaaaaaga
gacataaaaagcctcaccattcaagccgcacttcacatttggtatgaaaagtttatttttcttttagtgataatgtgatactatgacataatttcttggt
atttttagtttaaagtgtactagaaagtttagaaaataattgcaagatatggcaagaagggggatttaacacttaaattattttagttttctttgagtat
catttccatgtacttaaatgtatgttatatatcttctagtggttcatgacaatagcactttaccattacatattattctagaatttcagtgattgtgtgt
ctgtgtatgcatgagtgtaggcatgttacattacagtcttcagtatattttgcagcatatagaaaagggtaaattttaattgacatacaagcaggataca
acagtaggtacagaacttacaatttatacagttaaatacaagttcaaaaaatacatggctgtgtggggaattttacattagaataatattaatgttggtg
ttatgacttagggaggagataatagcaggatgttaagtgtgaaagctgatgtgttttgagaattgatgacttgcttaagggagctgttagaggttaatag
gtt
3' EST: CX972934 [+]aataaacagagaggtttcttttacttagtaattaaaaatcaacacagagagatggtttgtaaaaacatgatacaaactgttctttatacaatagaattct
tttgcatttataaacaatgccatataagattcagccgaatactgaacaggaatcatccttaatttgcatattcaaatttgaaaaccattaaacacattac
atcatgtgttaaacaaaaaaagttgcatacagttgtccagagctctaaagtcacaaaactttgagggtacagacttggcctaaccccaagcaaatcttgg
attcagtgcatcctgacttacgactgcacttttccaggcagctttgatgccatattcactagaacacagtgaccaaaaatatacagaaaaaatgtacata
tttaatttacataaagggtttttaaaatacattaacttgacaagattaatacaaaataaatacatagtaatccttccatttaaagccttaagcttttagc
ttaaaattgtactacttcaaaaaacggatgtttcaatgcttcttccagcgttattcttttagcggggtcgtactccaacattctttggataagatcaaat
agctgatgatgatccttgttatcagaaaccatgtattccttcaatggcttacaacgcttcttaacatatcggccagcagaactatgttcatcccaatcca
gccgatcaagataaaaatacttgtgtttcctagttttcttaatcatatggagtggtaaaggtcccagtat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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