XB-CLONE-3553704
Clone Name: IMAGE:7714271
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Gene:
uroc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX972983
[+]
gacgctgtgaagaaacagtacaacgataacattcgatggattcgagaagctggaaaacatgatctggttgtagggtcccaggctcgaattctgtattcag
accagaagggaagagtgtccatcgctgtggctatcaacagggctgtggctgcaggacgggtcaagggtcctgtggtgattagtcgggaccatcatgatgt
cagtggcacagacagtccctacagagaaacttccaatatctatgatgggtccgccttttgcgcagatatggccgtgcagaactttgtgggagactcattc
cgaggagcgacctgggttgctctgcataatggaggaggtgttggctggggtgaggtaattaatggaggatttggcctggttctggatggttcccaggagg
ctgaggagagagctacaaggatgctgagctgggatgtttccaatggggtggcgaggcgctgctggtcggggaaccaaaatgcctatgatacaattgtccg
aaccatggaagagaaccggaatctcaaagtgactttgccccatgcagtgaaggacaaaagtgttctggctttggctctgtcccttaattaacactcctat
tccatctgtctacctcttatgctatagaaatcacgttattaagcatcctcttccactcattatataatctagcacgtgtcaatcatcattttatattgtt
gtgcatcagaagggacgctcagtgcatggattacttatgcatttatggtctaactcccccagtgaacccactccagttagtctagatctataatgcctgt
gcacatactgg
3' EST: CX972982
[+]gccagtatgtgcacaggcattatagatctagactaactggagtgggttcactgggggagttagaccataggtgcataagtaatccatgcactgagcgtcc
cttctgatgcacaacaatataaaatgatgattgacacgtgctagattatataatgagtggaagaggatgcttaataacgtgatttctatagcataagagg
tagacagatggaataggagtgttaattaagggacagagccaaagccagaacacttttgtccttcactgcatggggcaaagtcactttgagattccggttc
tcttccatggttcggacaattgtatcataggcattttggttccccgaccagcagcgcctcgccaccccattggaaacatcccagctcagcatccttgtag
ctctctcctcagcctcctgggaaccatccagaaccaggccaaatcctccattaattacctcaccccagccaacacctcctccattatgcagagcaaccca
ggtcgctcctcggaatgagtctcccacaaagttctgcacggccatatctgcgcaaaaggcggacccatcatagatattggaagtttctctgtagggactg
tctgtgccactgacatcatgatggtcccgactaatcaccacaggacccttgacccgtcctgcagccacagccctgttgatagccacagcgatggacactc
ttcccttctggtctgaatacagaattcgagcctgggaccctacaaccagatcatgttttccagcttctcgaatccatcgaatgttatcgttgtactgttt
cttcacagcgtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: