XB-CLONE-3553526
Clone Name: IMAGE:7714337
|
Gene:
cdc73
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX973112 [+]
gaggaagaacagactccagctgtacagcctgtgccaagaccagtttcccaggcaaggccacctccaaatcagaaaaaaggatcacgcacgcccatcatca
ttatacctgctgctaccacttctttaattactatgttaaatgctaaagaccttcttcaagatctcaagtttgtttcatcagaagaaaagaagaaacaagg
ttgccagagggataatgaaaccttaatacagaggagaaaagaccaaatgcagccaggggggacaaccataagtgttacagttccatacagagttgtggac
cagccactaaagctgcttcctcaggactgggatcgtgttgtggcagtgtttgttcaaggtccagcctggcaattcaaaggctggccgtgggtgttgcctg
acggctcgcctgtagatatatttgcaaaaattaagggtttccacctaaagtacgacgaagttaggcttgatcccaatgtgcaaaaatgggatgtgacgct
tctggaattaagttatcacaaacggcacttggacagacctgtgtttttgaagttctgggagactttggacaggtatatggtgaaaaacaaatcccattta
agattctgaaaagcttgggttctctcatttctccatggaaagaaagagttgtgacttcacctccatctagagattaggactctattcacatttgtccaat
gaacctaccttgaatataccaggattgtcacaatttttttggacaatttgccatcatttacaaaaaaaggatagcatactattttttanaaaaaaggttt
gcaatgtttgcaattcaaatttgtatatttgatagtagggagagcatttgttttaacaaatacttcttttttttcccctt
3' EST: CX973111 [+]aaaaattttcataacagtattgtgtgtctaccattttattagaaacacaaagaatatcactttaatccacatacaaagctgtatgcttctcttcacactg
cccaccaatcagcacgcccaatgccatatggaacggatttggcaaatgtgtaagctcaacagcacaaaagagttctttatggtaatgaactaaattccaa
aaaaaaaaggaaaagaaaaaaaaaagtacttgagagaatgtaacatttacatacacataagtatactgtatttgaaaaggggaaaaaaaagaagtatttg
tttaaaacaaatgctctccctactatcaaaatatacaatttgaattgcaaacattgcaaaccttttttttaaaaaatagtatgctattcctttttttgta
aatgatggcaaattgtccaaaaaaattgtgacaatcctggtatattcaaggtaggttcattggacaaatgtgaatagagtcctaatctctagatggaggt
gaagtcacaactctttctttccatggagaaatgagagaacccaagcttttcagaatcttaaatgggatttgtttttcaccatatacctgtccaaagtctc
ccagaacttcaaaaacacaggtctgtccaagtgccgtttgtgataacttaattccagaagcgtcacatcccatttttgcacattgggatcaagcctaact
tcgtcgtactttaggtggaaacccttaatttttgcaaatatatctacaggcgagccgtcaggcaacacccacggccagcctttgaatgccaggctggacc
tgaacaacactgccacacacgatcccagtctgaggagcagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: