XB-CLONE-3553380
Clone Name: IMAGE:7714162
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Gene:
ctrc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973219 [+]
aggcgcccccaaagttaccagggttgtcggtggagaagatgtagtgccacacagttggccatggcagatctccctccagtatcagggaaccagtggtgct
tggggtcacacctgcggcggaaccctcatctctgaacagtgggtcctgactgctgcacattgtatcagctctggccgtgtctacagagtgttcctgggca
agcacagcctgcagcaggatgaggctgaagccgtcgctatctcccctgagaaaatcattgtccatgagaagtggaattcccttttcattatcaacgacat
tgctctgatcaagctgtcccagcctgctcctctgagcgaagcgatccaaccagcctgcattccttccagcggtgccatcctcgccaacgacttcccctgc
tttgtgactggatgggggcgcctctacaccaacggaccaatcgctgacaacttgcaacaagcccttctgcccgttgtggaccatgccacttgcaccctgc
gtgactggtggggaagccaggttcagaccaccatggtctgtgctggtggtgatggaattgtgtctggctgcaatggtgattctggtggccctctgaactg
ccaggcagcaggtggagcttgggaagtccacggcattgtcagcttcggctctggcattagctgcaactatgccaagaaaccaaccgtcttcacccgtgtg
tccgcctataacgactggatcagcgagaaaattgccagcaattaagaacaagcgcaatttccatcgtatccgcaacaacagggcatctgaatcgtgcatt
cctcgtatttcctttaacacctcgtgtcatacaca
3' EST: CX973218 [+]gcgagaggcgcgaaatccaaaatgtgtatgacacgaggtgttaaaggaaatacgaggaatgcacgaatcagatgccctgttgttgcggatacgatggaaa
ttgcgcttgttcttaattgctggcaattttctcgctgatccagtcgttataggcggacacacgggtgaagacggttggtttcttggcatagttgcagcta
atgccagagccgaagctgacaatgccgtggacttcccaagctccacctgctgcctggcagttcagagggccaccagaatcaccattgcagccagacacaa
ttccatcaccaccagcacagaccatggtggtctgaacctggcttccccaccagtcacgcagggtgcaagtggcatggtccacaacgggcagaagggcttg
ttgcaagttgtcagcgattggtccgttggtgtagaggcgcccccatccagtcacaaagcaggggaagtcgttggcgaggatggcaccgctggaaggaatg
caggctggttggatcgcttcgctcagaggagcaggctgggacagcttgatcagagcaatgtcgttgataatgaaaagggaattccacttctcatggacaa
tgattttctcaggggagatagcgacggcttcagcctcatcctgctgcaggctgtgcttgcccaggaacactctgtagacacggccagagctgatacaatg
tgcagcagtcaggacccactgttcagagatgagggttccgccgcaggtgtgaccccaagcaccactggttccctgatactggagggagatctgccatggc
caactgtgtggcactacatcttctccaccgacaaccctggntactttggggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: