XB-CLONE-3553367
Clone Name: IMAGE:7714402
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Gene:
luc7l3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973229 [+]
tcgcacgaggcaaggagcagagaccggaagagaagcaggagcagagagcgccaaagaagccaaagccacagcagaacagaaagaaagcacagatcacgaa
gccgggaaaagaggaggtctaaaagcagagaacgtaaatcttacaaacacaaatctagaagcagggaaagagaccgcgacagaaaatctaaagaaaaaga
aaaacgaagctcagatgacaaaaaaagcagagaatccagcagtcgagaaaaacagagtgactctagaaaaactgaatcgagggagtcggttatacaggtt
gaggtcaatggggcgaacgaaggcaataaatctgaaggtgacactcagtccaattaaaactgatctgatttgacctctgatcaggcagaggtaagtgcaa
catgttactcttggcttggtcttttgttttgctagaatgtaacggtgcagagtaagtatgcacagatgaagatggggcagatgatttagaagacagaaca
gcctccagtgagagaatgcaatgtagtcctgcaaaatatgtggaggtatactgttttcttctgccttttctttttgtagtgaaataacactcatccagtc
tctactccccatttctctcccccacaacgtgccttcacggaagcattagctacgcctctccaaacttaaataccactggagaattttccttgtttgagtt
gcttggtattttatcataatctacacatgtaaataagaattgaaatcctgaaatgcagtccattttagtcctttggcagtcccatccatttgatacgcac
gttaccttaag
3' EST: CX973228 [+]gagttacagaacaaactgcattagagtgcatggcaacaaagcaaaactctacacacatctgtcacaaaagcaaaaagctgagtagttttggtttttaaag
atacctagggctttaaggtaacgtgcgtatcaaatggatgggactgccaaaggactaaaatggacttgcattcaggatttcaattcttatttacatgtgt
agattatgataaaataccaagcaactcaaacaaggaaaattctccagtggtatttaagtttggagaggcgtagctaatgcttccgtgaaggcacgttgtg
ggggagagaaatggggagtagagactggatgagtgttatttcactacaaaaagaaaaggcagaagaaaacagtatacctccacatattttgcaggactac
attgcattctctcactggaggctgttctgtcttctaaatcatctgccccatcttcatctgtgcatacttactctgcaccgttacattctagcaaaacaaa
agaccaagccaagagtaacatgttgcacttacctctgcctgatcagaggtcaaatcagatcagttttaattggactgagtgtcaccttcagatttattgc
cttcgttcgccccattgacctcaacctgtataaccgactccctcgattcagtttttctagagtcactctgtttttctcgactgctggattctctgctttt
tttgtcatctgagcttcgtttttctttttctttagattttctgtcgcggtctctttccctgcttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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