XB-CLONE-3553192
Clone Name: IMAGE:7714422
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Gene:
hadh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973369 [+]
ctacttgcgcgctgcatgtcttcatctacggctgcagccaagaagctcgccatcaaaggtgtgacagtgatcggaggcggcctgatgggagccgggatag
cgcaggtagctgcatctacaggtcacacagttgtgctggttgatcagacagatgacatcctaaaaaagtctagaaaaaccattgaagacagcttgaaaag
agtaacaaagaagatgtttgcagataagcctgaggctgcctcccagttcattgaaaaaatccttgccaactttacaaccagcacagacccagcagctgtt
gtgcacagcactgaccttgtggttgaagccattgttgagaatttggacgtgaagaatgagctcttcaaaagactagataaatttgcccctgaacatacaa
tatttgctagcaacacctcatcattgccaattacggatatagcaaattccacaaccaggcaggaccggtttggtggactgcatttctttaaccctgtgcc
aatgatgaagcttattgaggtcataaagactccaatgacaagccaaacaacctttgagtccctcatggattttagtaaagctcttggaaaaacaccagtg
tcatgcaaggacacccctggctttattgttaaccgcctccttgtgccatacctaatggaatctgtcagactttatgagcgagggcatgcatcanaagaag
acattgatgttgcaatgaagcttggagcaggctatcccatgngtccttttgagctgctggattacgttggccttgacactagtaaatacattattgatgg
gtggcatgcgttgg
3' EST: CX973368 [+]agaagttttccttaaggcattagaaaaggattctgagccgcagtgaacaacccttgctccaaggcactgcggagtgatacgatgggaatcaaaaaatcag
atcaatagaattttctgttatgtagcgagagagcaacagctattctgtgttaattagcaagacagacctgacctcccctcccacagtcgccggacagtta
caaaatagagcgtgtggcattactttcatttgtatttataaaatccttctcccgtcttcttgccaagtttcttctcagcaaccagcttatctaacaattc
actaggcgcaaataacggattgttcggttccaacgcatgccacccatcaataatgtatttactagtgtcaaggccaacgtaatccagcagctcaaaagga
cccatgggatagcctgctccaagcttcattgcaacatcaatgtcttcttttgatgcatgccctcgctcataaagtctgacagattccattaggtatggca
caaggaggcggttaacaataaagccaggggtgtccttgcatgacactggtgtttttccaagagctttactaaaatccatgagggactcaaaggttgtttg
gcttgtcattggagtctttatgacctcaataagcttcatcattggcacagggttaaagaaatgcagtccaccaaaccggtcctgcctggttgtggaattt
gctatatccgtaattggcaatgatgaggtgttgctagcaaatattgtatgttcaggggcaaatttatctagtcttttgaagagctcatttctcacgtcca
aaatttctcacatggctttcacccacaggtcagtgctgtgcacaacagctgctgggtctgtgctggttgtaaaagtggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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