XB-CLONE-3553100
Clone Name: IMAGE:7714192
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973434 [+]
cacaggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaaggatacggtggaccag
atccagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaagccaagtaattc
cctttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtgaagatttctcc
ttacgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagccaactagagaag
aacactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatctttggcaccat
attcagaaaaactccaggcccaaatagatgagagagtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataagattggccatca
agtacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattccaaatgagaaagagt
gttgagcaagcagacacccaattaaaacagatcactgagcagttaaaagctcaactgagcccctatgcagatgatct
3' EST: CX973433 [+]atagggcgagaggttactgggggaggttcatcccagacataatgaccaagaaacaatggcagcagaattataaataaaatggaacattttagggctgtaa
gagaaacataaagctattcaggcacaacaaatgacagacataacacaaagacaaatctgtgttttacaaaacttgctacagtgtgcatttacttcacttt
tacattttcttcttacttgatttgcagtaggccgacagcccaatctctgggtatttttttttctttttatgattgatatggcatacaggtggtgcaagtt
gtaatggatactgtggtctgatgacaatttgggtgtatacgttgtttagttttcagtttcagttatacctttatttatgaagtcgctaaacttatctcgg
atgtctttttctaagtattccatttgatcttgcacagtcactgtgtatttcccaaatttcagtttcatgtcctctaccctctgcactagtgccttgttga
gtgcctctccatagggagtgatgctaactttaaattctgcaatcttctgctccatctgctggtttagctcggtgagatatggttctagtgtctgcttagc
atccctagcatttacctggagtttctctttcagatcatctgcataggggctcagttgagcttttaactgctcagtgatctgttttaattgngtgtctgct
tgctcaacactctttctcatttggaattccatgctctctatctgcttggctaaattctcctgcatctcctctgcataagggctcaggctctcacgtaact
tttgtacttgatggccaaatctatctctcacttcatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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