XB-CLONE-3552972
Clone Name: IMAGE:7714637
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Gene:
agfg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973529 [+]
caaactgtagcaaaacattagctttattatgcagagatgagtctttagtatggagattaggcgcatagaaatgggtagctttattgggcaaaataaaagt
ttgtcttttgtcaacaaataaaatttgtaataaagaccacattgcttttgcaatccccactccctttgaaaccagattgttaaaacaaattttttttttc
tttccattttaagtgatggatccggaacctgcttcagctctccagcatcgggtacggtgttaagagttagaaatactgatatgaataattaactagtccc
taagaaaatatatgtacagtactgcagcttaatgaatatggaatctgcaataacttaccaaatttaatatacatgatacgcctcaggttagacccttcgc
tgctgaagagctattgactctccgctctgctaggaaattaccagtcaaaccttcaggcagtgaagggttttgatgctaaaaggctttccaattgcacata
ttaccttgtttgtcctccttttttgtttaaatacaaaaaaatatatatataattgcaggctctgaaagaactccctgtgtttaaagcttacgctggttaa
acaagacaaacagtgcaagaggcagtacatgtacggtgatgcccggtaatctcctgtgttagtctaaggctataagaaagggttcgtggatgaacttcca
gttggaaactgccccgaaggtgccccggccatgaaaggattgctagagactccaggagctgcaacctggccaaacggagtaacacaggcttggtctgccc
aaatacagcaaaccctgcaccntg
3' EST: CX973528 [+]gcgcacctccaggggtacgagacggccacgcggcagaagccccattccatgctttccaccagacccgactagcgacagccgcaggagcattggatgtctc
ttcctttggcacagcatctctaagcatgcccgctggctttggaacacagacaccatacagtcttcctacaagctttagtgggagtttccaccaacctgcc
ttcgcccctcaaacagccttcccccaacagactgctttctcgcagcagcctaatgggaatatacaaggtgcagggtttgctgtatttgggcagaccaagc
ctgttgttactccgtttggccaggttgcagctcctggagtctctagcaatcctttcatggccggggcaccttcggggcagtttccaactggaagttcatc
cacgaaccctttcttatagccttagactaacacaggagattaccgggcatcaccgtacatgtactgcctcttgcactgtttgtcttgtttaaccagcgta
agctttaaacacagggagttctttcagagcctgcaattatatatatatttttttgtatttaaacaaaaaaggaggacaaacaaggtaatatgtgcaattg
gaaagccttttagcatcaaaacccttcactgcctgaaggtttgactggtaatttcctagcagagcggagagtcaatagctcttcagcagcgaagggtcta
acctgaggcgtatcatgtatattaaatttggtaagttattgcagattccatattcattaagctgcagtactgtacatatattttcttagggactagttaa
ttattcatatcagtatttctaactcttaacaccgtacccgatgctggagagct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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