XB-CLONE-3552903
Clone Name: IMAGE:7714643
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Gene:
ctrb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973575 [+]
caggttatgccaggattgtgaatggcgaggaagcagttccagggtcttggccttggcaagtctccctgcaggattccacttcatggcacttttgcggagg
ttcccttattaacaatgagtgggttgtaactgctgctcactgtggtgtctcgaccagagataaggtggttctcggagagcatgaccgaggctcaaacgtt
gaaaaaatccagtctcttgctgttgctaaggtcttcacacacccacagtggaattccaacacaatcaacaatgatatttccctgattaagctagccactc
cagctgtccttggcgcaactgtggctccagtttgtctggctaatactggagacgattatgaaggagggcgcatctgtgttaccagtggatggggaaagac
cagatacaatgcatttacaactccaaaccaactgcagcaaactgctctacctctgctgacaaacgatcagtgcaagagctactggggaaataatatcact
ggcacaatgatctgcgctggtgctgctggctcctcttcctgcatgggtgattctggtggaccacttgtatgccaggctaatgatgcatggacacttgttg
gcattgtgtcctggggaagcagtatgtgctcaacaagcactccagctgtgtacgcccgtgttgcagttctccgttcttgggttgacgagattgtggcttc
caactaatgcacattccaaaatatagtattctcaaaatggtggagagaaattaaattgtgaaatgtaatgtaactgtctgaaaatatcagtact
3' EST: CX973574 [+]ccagtactgaatatttcagacagttacattacatttcacaatttaatttctctccaccattttgagaatactatattttggaatgtgcattagttggaag
ccacaatctcgtcaacccaagaacggagaactgcaacacgggcgtacacagctggagtgcttgttgagcacatactgcttccccaggacacaatgccaac
aagtgtccatgcatcattagcctggcatacaagtggtccaccagaatcacccatgcaggaagaggagccagcagcaccagcgcagatcattgtgccagtg
atattatttccccagtagctcttgcactgatcgtttgtcagcagaggtagagcagtttgctgcagttggtttggagttgtaaatgcattgtatctggtct
ttccccatccactggtaacacagatgcgccctccttcataatcgtctccagtattagccagacaaactggagccacagttgcgccaaggacagctggagt
ggctagcttaatcagggaaatatcattgttgattgtgttggaattccactgtgggtgtgtgaagaccttagcaacagcaagagactggattttttcaacg
tttgagcctcggtcatgctctccgagaaccaccttatctctggtcgagacaccacagtgagcagcagttacaacccactcattgttaataagggaacctc
cgcaaaagtgccatgaagtggaatcctgcagggagacttgccaaggccaagaccctggaactgcttcctcgccattcacaatcctggcataacctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: