XB-CLONE-3552851
Clone Name: IMAGE:7714791
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Gene:
btrc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973609 [+]
tgatatctcgaatgtgccactatcagcatggacatataaatacataccttaagccaatgttacaaagggatttcatcaccgcactaccagcccgcggact
agatcacatagcagaaaacatactatcatacctggatgcaaggtctttgtgttccgcagaactggtatgtaaggagtggtaccgagtgacctcagatgga
atgctctggaagaagctcatagagaggatggttaggacagattctctttggaggggactggcagaaagaagaggatggggtcaatatctatttaaaaaca
aacctccagatgggaaaacaccaccaaattcattctacagagcactttacccaaaaattattcaagacatagagacaatagagtccaactggcgctgtgg
gagacacagcttacaaagaattcactgcaggagtgaaacaagcaaaggggtgtactgcctgcagtacgatgatcagaagatagtaagtggactcagagat
aacaccataaagatctgggataagaatactttggagtgcaaacgagtgctgatgggccacactgggtcagttctctgcctgcagtatgatgagagagtaa
tcataaccggctcttctgactctaccgtccgggtttgggacgtaaacacaggagaaatgttgaacactctgattcaccactgtgaggctgtgctacattt
gaggtttaacaatggtatgatggtcacctgctccaaagatcgttccattgcagtgtgggacatggcctctgccacagatatcacattacgaagagttcta
gtaggccaccgagctgcccgtaat
3' EST: CX973608 [+]ttttgtatagcagaaaaataaatattttgcattttacggattaagcggttttgtggcgctagtagggcagtcggatagctgcagtaatgcacaaggaagt
tctctagtgagccatcaaatcctttgaaggtgccattctggggcaagataaagtcattatttatgaaagacaagaattctgacttctgcattaggataat
gaatatgatcaaaatctgctatcatgaagcctaaagcttagtttcccttcatccatgattaaactggaagtttggcatagttttatatcatttctgtatt
tttatttcataggaatacatatacactaaatgggatcatgggaacctgcattggaaagcatggctccctacccataggccaacaatagccaatataaagg
aatttaataggagcacagacaccaatgtcaaacaatttctttacatgatcacaatcctaaacactaactgaattataaaaaaaaaaaaaatctatgcact
gtggtgatttcgagagcagatttttacagtgtctctttaaaaaaaacaaacaaacccccacaagtttggctttaagtaaaggacagaaaacgagtgcttt
ttttttttcttcttctaaaggacaaaaagatactttcctgttgcgttacgttaagtgtttgtacctcagttttcagatcatgcagaatatttaatagtgg
ttcacatgcaagaagagatgagtttaaatacgtatttacgaagagcgctatggcaaatagggctgcccgcgaagatcagaagcatgagttagtagtcgtg
tgcagaaaatatacatggtgttttgtgaacgtcagtacaagcgcggtgacggaaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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