XB-CLONE-3552826
Clone Name: IMAGE:7714507
|
Gene:
etnppl
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX973627 [+]
ccgaggaacaatctagtttatttgactagatgtgcagaatttaatgaataagcttttatttcttattatgttttgaatctagtttatagagaacaaatga
tatactgtagatatgcatggcatggatttagccaagcagtaggatctggtataatgtgattatggatatctgctcttctggcactctttcatagtcattc
ttgtcaagtgctgttatacatctgaaatgttctgacactttaagaaaagaagcatacgcatgtttacctagttttaagaaaactgctgatcccccatgta
gcttctcattttaaaatataaaacattatagcaccttgtatgttgacaaaaaagaaaataccataggcagtgagaaaatgcccccttatgctattctatg
gggctcatattcaagtaaaagggatataggaatcatgtacacagggatttcaggctgctacatcagatctgtagggacccctaataaaaagtaaaataat
gtagcgagtggaaaagggttacttatttgagcaagagctaaaatcttggtgtatctaaacagttggaaacggcttaaaattttcttgtacatagcatgct
cagtacattcagcatgctgaggtcttttaaactgcatcttaaatctaagccattaaaacatgggaaaagattccattccaatatgatatactgaaactgc
aaatacagaataaaaaaaaataaaaaggatgcaccacataaacccctaaaattcattggcaaatcaaatttaacaacccttcgccagactagaccaagca
tcttcatttgttagagttgttagaaatagaagggcagcacagtttgaagattctgaactc
3' EST: CX973626 [+]gtggatataatttattatagacaattgtgtttagtcctttattaacaaaaaacattttctataaataatttaatttttttccagctagcgaaagtgaaag
tcttttgtactgaccacaggaggataaacttagatctgatatcttcttttatatagattgctgcttttattgctgagtccatgcagagttgtggaggtca
aataatcccaccagcaggctacttccagaaagtgtcagagtatgtacagagcctaatgagaaataatggcttttctcggttcacttaatctagcttttct
atcttatagcacattaaactcatacagtccaaacaattacaaaacatataggagctgaattactaacagaagttctaaactgcactagagcagttgcaaa
tagcagccaatcagcaatttgttttaatcagtctactacaagttagaatatgaaagcaaaggtctggtgatatttagcttctttgttagtaagtcagccc
cataattgtgacactattgtgagattatactattcctacagaatgcaatacaaacttggtgtagacatttattgtatacaaacacaatgttaagaagaag
aaactttccaatcattcattgtcttttttatgtttaccttcattctcttgactagtttctatacatctttctaccaaccttttcagaattcctatgtttc
ctctctctctctctctctctgtttttatatcatcatcattgtctggaaataggtctgcaaagctcattcactaatatgtgcaagataccatataagtcta
gtttcacacttctaaagatgtgtaaaaagtgaatttctgccttttattccatagtgtgcatctaatctgagcagtttgtcacacctacacttacatgtgt
cacatgag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: