XB-CLONE-3552776
Clone Name: IMAGE:7714559
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Gene:
cpo.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX973660 [+]
catacttctctgcatttgtattttaggcattctaacacaggttggggcatacttaaaggtccagtacaatggagatcaggttttgaagctcaccctgaaa
acaacagatgactgtgagcacatgcaaaatatctgcaaacaactccagcttgatctatggaagccaagcagaatagaagatattcaacctgggaaagaaa
tgcatgttcgaataccttttccacttcttcaaaaattcaaagagaaccttcatcaatattctattccattcgaagtcatgataaaagatgtacaaaaact
tatagacagcagcaatgtgggtgactacagaagacagaaaaaaatattggctgaatttgattataccacatatcaccccatggatgagatttatcagtgg
atggatcaagttaaagaggcatatagtgacctggtttctatgcattacctaggatcaacatatgagctaaggcctatttactatttcaaaattggctggc
cttctgacaaacagaagaagatcatttggatggactgtgggatacatgctcgtgaatggattgccgttgcctattgccaatggtttgttaaagagattct
tgaaactcacaagacaaatcctttgctccagaaagtactccataacatagacttctatattgtacctgtcctaaatattgatggatttgtttattcctgg
aatgtgaatcgtctttggcgaanatcgcggtctcctcataancatggatcctgctacggggtggaccttaatcgaaattttaattcaaaatggggcagta
ttggcgcatcttaacattgtagagatgaaacatactgcggaactgggactgcatctg
3' EST: CX973659 [+]tgacccagaattgctatatagtaagtgtgatgtagatccaactctgtactcggtgccatgcttctcttggagttttgcagcagccttctgagcaacattt
atcatctcttcatgatttatagaaggatcctttgtataaccatagggcagaagaagaagttgaccataggaatgaattgttaggaaacacaaaacatcag
atttaagactccccagcagcttagatacagcattaacttctggttcagatgcaggtccagttccgcagtatgtttcatctctacaattgttagatgcgcc
aatactgccccattttgaattaaaatttcgattaaggtccaccccgtagcaggatccattgttatgaggagaccgcgattttcgccaaagacgattcaca
ttccaggaataaacaaatccatcaatatttaggacaggtacaatatagaagtctatgttatggagtactttctggagcaaaggatttgtcttgtgagttt
caagaatctctttaacaaaccattggcaataggcaacggcaatccattcacgagcatgtatcccacagtccatccaaatgatcttcttctgtttgtcaga
aggccagccaattttgaaatagtaaataggccttagctcatatgttgatcctaggtaatgcatagaaaccaggtcactatatgcctctttaacttgatcc
atccactgataaatctcatccatggggtgatatgtggtataatcaaattcagccaatatttttttctgtcttctgtagtcacccacattgctgctgtcta
taagtttttgtacatcttttatcatgacttcgaatgga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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